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Mycoplasma gallisepticum Edward and Kanarek


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Bacterial type strain that was isolated from a suspension of tracheal and air sac tissues of chickens with chronic respiratory disease. This strain has applications in quality control, media testing, and agricultural research.
Product category
Strain designation
[NCTC 10115, PG 31, X95]
Type strain
Genome sequenced strain
Isolation source
Suspension of tracheal and airsac tissues of chickens with chronic respiratory disease
Agricultural research
Media testing
Pharmaceutical testing
Quality control
Product format
Storage conditions
2°C to 8°C
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ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information


Specific applications
Control strain
Media testing
Pharmaceutical and Personal Care
Recommended as a test microorganism for EP 2.6.7 Mycoplasmas
Recommended as a test microorganism for USP <63> Mycoplasma Tests

Handling information

95% Air, 5% CO2
Handling procedure
  1. Follow instructions as suggested for the culturing of Mollicutes:


    1. Open the vial according to the enclosed instructions.
    2. Rehydrate the entire pellet with approximately 0.25 mL of #243 broth. Aseptically transfer the entire contents to primary tube containing 2.25mL of #243 broth. Make serial dilutions by transferring 0.25mL from the primary tube to second to third tube, etc.
    3. Plates may be inoculated to check colony morphology.
    4. Incubate broth tubes at 37°C, aerobic, and the plates at 37°C 5% CO2 for 3-7 days.
    5. Depending on the medium used, growth will be indicated by increased turbidity, a color change, or both.
    6. Inoculate a Trypticase Soy Agar with 5% Defibrinated Sheep Blood plate with 0.1 mL to check for contamination. Incubate at 37°C, 5% CO2. A second plate may be incubated at 37°C aerobic to ensure no contaminants in either atmospheres.
    7. This strain starts to show growth in the first few dilution tubes within 72 hours. Additional incubation may be required for growth on solid medium.
    8. Subsequent, fresh transfers grow in 24-48 hours. The freeze drying process and the cryoprotectant occasionally slows the growth rate of the initial culture.
Handling notes
Store vials at freezer temperatures until ready to use.
Additional information on this culture is available on the ATCC® web site at

Quality control specifications

Verification method
Whole-genome Sequencing


Deposited as
Mycoplasma gallisepticum Edward and Kanarek
RG Wittler
Chain of custody
ATCC <-- RG Wittler <-- DG ff. Edward PG 31 <-- F Markham
Type of isolate

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.


This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.


Frequently Asked Questions


Curated Citations

Edward DG, Kanarek AD. Organisms of the pleuropneumonia group of avian origin: their classification into species. Ann. N.Y. Acad. Sci. 79: 696-702, 1960. PubMed: 13819382

Edward DG, Freundt EA. Type strains of species of the order Mycoplasmatales, including designation of neotypes for Mycoplasma mycoides subsp. mycoides, Mycoplasma agalactiae subsp. agalactiae, and Mycoplasma arthritidis. Int. J. Syst. Bacteriol. 23: 55-61, 1973.

. . Vet. Rec. 79: 580, 1966.

British Pharmacopoeia Commission Tests for microbial contamination. London, UK:British Pharmacopoeia Commission;British Pharmacopoeia Appendix XVI B, 2003

Skerman VB, et al. Approved lists of bacterial names. Int J Syst Bacteriol 30: 225-420, 1980.

View All Curated Citations for this Product

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