Acidiphilium acidophilum (Guay and Silver) Hiraishi et al.
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2. Withdraw 0.5 ml of #738 broth from a single test tube (5 to 6 ml) and rehydrate the vial contents.
3. Aseptically transfer this aliquot back into the broth tube. Additional broth and slant tubes may be inoculated with 0.3 ml each from the initial broth tube. Agar plates may be inoculated using 0.1 ml of the initial broth per plate.
4. Incubate tubes and plates at 26oC.
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Guay R, Silver M. Thiobacillus acidophilus sp. nov.; isolation and some physiological characteristics. Can. J. Microbiol. 21: 281-288, 1975. PubMed: 234784
Harrison AP Jr.. Genomic and physiological comparisons between heterotrophic thiobacilli and Acidiphilum cryptum, Thiobacillus versutus sp. nov., and Thiobacillus acidophilus nom. rev.. Int. J. Syst. Bacteriol. 33: 211-217, 1983.
Hiraishi A, et al. Phylogeny and photosynthetic features of Thiobacillus acidophilus and related acidophilic bacteria: its transfer to the genus Acidiphilium as Acidiphilium acidophilum comb. nov.. Int. J. Syst. Bacteriol. 48: 1389-1398, 1998. PubMed: 9828441
derived from a culture of Thiobacillus ferrooxidans strain TM