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Microbiome and Molecular Diagnostics Standards for Next-generation Sequencing-based Assays

DNA rods with bacteria.

Abstract

The complexities involved in 16S rRNA and shotgun metagenomic analysis methods pose significant challenges for microbiome research and frequently result in the introduction of biases. One of the primary obstacles in assay standardization is the limited availability of reference materials and robust analytical tools. To support this need, ATCC has developed mock microbial communities from fully sequenced and characterized ATCC strains, selected based on their phenotypic and genotypic attributes or relevance in disease-specific research. These NGS Standards mimic mixed metagenomics samples and offer a universal control for microbiome analyses and assay development.

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