ATCC ATCC Logo 0

Genomic DNA from Mycoplasma hyorhinis strain BTS-7 [ATCC 23234, D.G. ff. Edward PG 42, NCTC 10130]

qCRM-17981D

Quantified genomic DNA isolated from Mycoplasma hyorhinis strain BTS-7 [ATCC 23234, D.G. ff. Edward PG 42, NCTC 10130]. Bacterial genomic DNA is provided in 1X TE Buffer (pH 8.0).
Product category
Bacteria
Product type
Nucleic acid
Certified reference material
Molecular standard
Derived from
Mycoplasma hyorhinis BTS-7 [ATCC 23234, D.G. ff. Edward PG 42, NCTC 10130] (ATCC 17981)
Organism
Mycoplasma hyorhinis
Type strain
Yes
Applications
Assay development
Next-generation sequencing
Quality control
Mycoplasma testing
Specification range
≥ 1 x 106 to 1 x 107 genome copies/μL
Product format
Frozen
Storage conditions
-70°C or colder
Buy Now
Price: $578.00 EA
Discounts are available for our fellow nonprofit organizations.

Generally ships within 1-3 business days

Documentation

Haven't found what you need?

Customize solution

We tailor our materials for your specific application.

Get application-specific materials

Biosafety Icon BSL 1

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

Required Products

These products are vital for the proper use of this item and have been confirmed as effective in supporting functionality. If you use alternative products, the quality and effectiveness of the item may be affected.

Molecular Grade Water

60-2450

Price: $40.00 ea

Detailed product information

General

Specific applications
Quantitative external control produced under an ISO 17034 accredited process for use in testing and calibration in ISO 17025 accredited laboratories, inclusivity/exclusivity testing, establishing limits of detection, verification or comparison of test methods, and other molecular applications.

Characteristics

Comments
Genome copy number is based on the quantification of the 16S rRNA gene from nine averaged samples. Mycoplasma hyorhinis (ATCC 17981) has one copy of the 16S rRNA gene per cell.

The reported genome copy number may vary depending on quantitation method used.

The limits of detection for this nucleic acid preparation will depend on the set of primers used. Primer efficiency should be determined empirically.

This preparation of high molecular weight DNA is appropriate for use in the polymerase chain reaction (PCR)* process and other molecular biology applications.
*The PCR process is covered by patents owned by Hoffmann-La Roche Inc. Use of the PCR process requires a license.

Droplet Digital™ PCR is a trademark of Bio-Rad Laboratories, Inc.

Handling information

Handling procedure
  1. Thaw the vial at room temperature.
  2. Avoid exposing genomic DNA to repeated freeze-thaw cycles. Subjecting genomic DNA to repeated free/thaw cycles may result in degradation of the DNA and variations in copies/µL.
Handling notes
This preparation of high molecular weight DNA is appropriate for use in the polymerase chain reaction (PCR)* process and other molecular biology applications.
*The PCR process is covered by patents owned by Hoffmann-La Roche Inc. Use of the PCR process requires a license.
Droplet Digital™ PCR is a trademark of Bio-Rad Laboratories, Inc.

Quality control specifications

Integrity
Integrity of DNA was determined by electrophoresis on a 1% agarose gel stained with ethidium bromide.
Functional tests
Functional activity was confirmed by PCR amplification of the 16S ribosomal RNA gene.
Identity
Identity confirmed by sequencing of 16S ribosomal RNA gene.
Quality accreditation

Manufactured under an ISO 17034 accredited process

    ACLASS Accredited Reference Material Producer AR-1384

History

Depositors
ATCC
Special collection
Certified Reference Materials
Cross references
GenBank M24658 M. hyorhinis, 16S ribosomal RNA small subunit.
GenBank AF294993 Mycoplasma hyorhinis 16S-23S ribosomal RNA intergenic spacer, complete sequence; and 23S ribosomal RNA gene, partial sequence.
GenBank GCA_000383515.1 Mycoplasma hyorhinis ATCC 17981 Assembly

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.

Certified Reference Material produced under an ISO 17034 accredited process.

Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

References

Frequently Asked Questions

Need assistance with this product? Contact our Technical Support team.

Telephone

Telephone

US and Puerto Rico
800-638-6597

Outside the US
+1-703-365-2700
hours

Hours of Operation

Monday-Friday
9:30am - 5:30pm
US Eastern Time