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HCM-CSHL-0248-C19

PDM-257

Product category
Human cells
Product type
Cell model
Organoid
Organism
Homo sapiens
Morphology
organoid
Tissue
Large intestine; Rectum
Disease
Malignant neoplasm
Applications
3D cell culture
Cancer research
Product format
Frozen
Storage conditions
Vapor phase of liquid nitrogen
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Price: $3,328.00 ea
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Documentation

Biosafety Icon BSL 1

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Required Products

These products are vital for the proper use of this item and have been confirmed as effective in supporting functionality. If you use alternative products, the quality and effectiveness of the item may be affected.

CellMatrix Basement Membrane Gel

ACS-3035

Price: $221.00 ea

Stem Cell Freezing Media

ACS-3020

Price: $55.00 ea

ROCK Inhibitor Y27632

ACS-3030

Price: $211.00 ea

Detailed product information

General

Specific applications
Basic research, compound screening.

Characteristics

Cells per vial
≥ 1.0 x 106
Volume
1.0 mL
Growth properties
Embedded 3D culture
Clinical data

ICD-10-CM code: C19, malignant neoplasm of rectosigmoid junction

See associated clinical data for patient profile information, if available.
https://portal.gdc.cancer.gov/  
https://hcmi-searchable-catalog.nci.nih.gov/model/HCM-CSHL-0248-C19
Comments
Next-generation cancer model from the Human Cancer Models Initiative (HCMI). Refer to the following websites for additional information on this model including protocols, clinical information, and bioinformatics data.

https://ocg.cancer.gov/programs/hcmi/resources
https://portal.gdc.cancer.gov/  
https://hcmi-searchable-catalog.nci.nih.gov/model/HCM-CSHL-0248-C19


If use of this culture results in a scientific publication, it should be cited in that manuscript in the following manner: HCM-CSHL-0248-C19 (ATCC® PDM-­257).

Additionally, please acknowledge the HCMI as follows: “We used models and data derived by the Human Cancer Models Initiative (HCMI) https://ocg.cancer.gov/programs/HCMI; dbGaP accession number phs001486.”

Handling information

Unpacking and storage instructions
  1. Check all containers for leakage or breakage.
  2. Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below ­-130°C, preferably in liquid nitrogen vapor, until ready for use.
Complete medium

To prepare the complete medium for this organoid please refer to the Organoid Media Formulation #1.

ATCC offers the recombinant proteins, small molecules and other supplements to make this complete medium; CoreKit 1A (ATCC ACS-7100) provides these supplements in convenient, pre-portioned, ready-to-reconstitute format that does not require aliquoting or storage once prepared.

Temperature
37°C
Atmosphere
95% Air, 5% CO2
Handling procedure
Seeding density: We recommend seeding this model at 0.25 - 1 x 106/ viable cells in 100 µL of ECM per well of a 6-well plate. 

ECM: We recommend culturing this model in ATCC CellMatrix (ATCC ACS-3035) or Corning Matrigel. Include 10 µM ROCK Inhibitor Y-27632 (ATCC ACS-3030) in medium for the first 2-3 days following subculture. 

For a brief overview of the thawing procedure see our quickstart guide Thawing Cryopreserved Human Organoids.

Subculturing procedure

Initiating culture from frozen vials: For a brief overview of the thawing procedure see our quickstart guide Thawing Cryopreserved Human Organoids.

Seeding density: 0.25 - 1 x 106/ viable cells in 100 µL of ECM per well of a 6-well plate. Alternatively, split at 1:2-1:4 every 7-10 days. For example, collect organoids from 100 µL of extracellular matrix (ECM) from a single well of a 6-well plate and re-seed into 2-4 wells of a 6-well plate in 100 µL ECM per well. 

Media renewal: Perform a complete medium change every 2-3 days. Include 10 µM ROCK Inhibitor Y-27632 (ATCC ACS-3030) in medium for the first 2-3 days following subculture. 

For a brief overview of the subculture and expansion of organoids see our quickstart guide Subculture and Expansion of Human Organoids Protocol.

For more details on the handling and culture of organoids see our methods paper in Current Protocols in Cell Biology

Reagents for cryopreservation
We recommend cryopreserving this model in ATCC Stem Cell Freezing Media (ATCC ACS-3020).
Cryopreservation

For a brief overview of the cryopreservation procedure for organoids see our quickstart guide Organoid Cryopreservation Protocol.

For more details on the handling and culture of organoids see our methods paper in Current Protocols in Cell Biology.

Quality control specifications

Bacterial and fungal testing
Not detected
Mycoplasma contamination
Not detected
Virus testing
Hepatitis B virus (HBV): Not detected
Cytomegalovirus (CMV): Not detected
Human Immunodeficiency virus (HIV): Not detected
Epstein-Barr virus (EBV): Not detected
Human papillomavirus (HPV): Not detected

History

Depositors
Cold Spring Harbor Laboratory
Year of origin
2018

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Material Transfer Agreement Addendum for Organoid Products

For every order of this item, you must provide a signed Material Transfer Agreement Addendum for Organoid Products. We cannot ship this item until we receive this addendum. Email the signed addendum to [email protected] with a reference to both your account and sales order numbers. Once received, your addendum will be reviewed, and this item will be released for shipment if all requirements are met. If you need assistance with your order, please contact our Customer Care team or your applicable distributor.

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

References

Frequently Asked Questions

Need assistance with this product? Contact our Technical Support team.

Telephone

Telephone

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Outside the US
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