Basic research, compound screening
ICD-10-CM code: C25 malignant neoplasm of pancreas; cancer
See associated clinical data for patient profile information, if available.
Next-generation cancer model from the Human Cancer Models Initiative (HCMI). Refer to the following websites for additional information on this model including protocols, clinical information, and bioinformatics data.
If use of this culture results in a scientific publication, it should be cited in that manuscript in the following manner: HCM-CSHL-0182-C25 (ATCC PDM-203™).
Additionally, please acknowledge the HCMI as follows: “We used models and data derived by the Human Cancer Models Initiative (HCMI) https://ocg.cancer.gov/programs/HCMI; dbGaP accession number phs001486.”
To prepare the complete medium for this organoid model please refer to the Organoid Media Formulation #3.
ATCC offers the recombinant proteins, small molecules and other supplements to make this complete medium; Organoid Media Kit 1B (ATCC ACS-7101) provides these supplements in convenient, pre-portioned, ready-to-reconstitute format that does not require aliquoting or storage once prepared.
Seeding density: We recommend seeding this model at 0.25 - 1 x 106/ viable cells in 100 µL of ECM per well of a 6-well plate.
ECM: We recommend culturing this model in ATCC Cell Basement Membrane (ATCC ACS-3035) or Corning Matrigel. Include 10 µM ROCK Inhibitor Y-27632 (ATCC ACS-3030) in medium for the first 2-3 days following subculture.
For a brief overview of the thawing procedure see our quickstart guide Thawing Cryopreserved Human Organoids.
Initiating culture from frozen vials: For a brief overview of the thawing procedure see our quickstart guide Thawing Cryopreserved Human Organoids.
Seeding density: 0.25 - 1 x 106/ viable cells in 100 µL of ECM per well of a 6-well plate. Alternatively, split at 1:2-1:4 every 7-10 days. For example, collect organoids from 100 µL of extracellular matrix (ECM) from a single well of a 6-well plate and re-seed into 2-4 wells of a 6-well plate in 100 µL ECM per well.
Media renewal: Perform a complete medium change every 2-3 days. Include 10 µM ROCK Inhibitor Y-27632 (ATCC ACS-3030) in medium for the first 2-3 days following subculture.
For a brief overview of the subculture and expansion of organoids see our quickstart guide Subculture and Expansion of Human Organoids Protocol.
For more details on the handling and culture of organoids see our methods paper in Current Protocols in Cell Biology
We recommend cryopreserving this model in ATCC Stem Cell Freezing Media (ATCC ACS-3020)
For a brief overview of the cryopreservation procedure for organoids see our quickstart guide Organoid Cryopreservation Protocol.
For more details on the handling and culture of organoids see our methods paper in Current Protocols in Cell Biology.
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