ATCC 2023 Holiday Shipping Schedule: We will be closed from December 22nd to January 1st. To ensure timely delivery, please submit orders in accordance with the following: Domestic orders no later than December 15th for shipment by December 20th 2023; International orders no later than December 12th for shipment by the first week of January 2024. We will resume our normal shipping schedule on January 2nd, 2024. Thank you for your cooperation.
ICD-10-CM code: C19 malignant neoplasm of rectosigmoid junctionSee associated clinical data for patient profile information, if available.
To prepare the complete medium for this organoid please refer to the Organoid Media Formulation #1.
ATCC offers the recombinant proteins, small molecules and other supplements to make this complete medium; CoreKit 1A (ATCC ACS-7100) provides these supplements in convenient, pre-portioned, ready-to-reconstitute format that does not require aliquoting or storage once prepared.
Seeding density: We recommend seeding this model at 0.25 - 1 x 106/ viable cells in 100 µL of ECM per well of a 6-well plate.
ECM: We recommend culturing this model in ATCC Cell Basement Membrane (ATCC ACS-3035) or Corning Matrigel. Include 10 µM ROCK Inhibitor Y-27632 (ATCC ACS-3030) in medium for the first 2-3 days following subculture.
For a brief overview of the thawing procedure see our quickstart guide Thawing Cryopreserved Human Organoids.
Initiating culture from frozen vials: For a brief overview of the thawing procedure see our quickstart guide Thawing Cryopreserved Human Organoids.
Seeding density: 0.25 - 1 x 106/ viable cells in 100 µL of ECM per well of a 6-well plate. Alternatively, split at 1:2-1:4 every 7-10 days. For example, collect organoids from 100 µL of extracellular matrix (ECM) from a single well of a 6-well plate and re-seed into 2-4 wells of a 6-well plate in 100 µL ECM per well.
Media renewal: Perform a complete medium change every 2-3 days. Include 10 µM ROCK Inhibitor Y-27632 (ATCC ACS-3030) in medium for the first 2-3 days following subculture.
For a brief overview of the subculture and expansion of organoids see our quickstart guide Subculture and Expansion of Human Organoids Protocol.
For more details on the handling and culture of organoids see our methods paper in Current Protocols in Cell Biology
We recommend cryopreserving this model in ATCC Stem Cell Freezing Media (ATCC ACS-6000). For a brief overview of the cryopreservation procedure for organoids see our quickstart guide Organoid Cryopreservation Protocol.
For more details on the handling and culture of organoids see our methods paper in Current Protocols in Cell Biology.
For every order of this item, you must provide a signed Material Transfer Agreement Addendum for Organoid Products. We cannot ship this item until we receive this addendum. Email the signed addendum to [email protected] with a reference to both your account and sales order numbers. Once received, your addendum will be reviewed, and this item will be released for shipment if all requirements are met. If you need assistance with your order, please contact our Customer Care team or your applicable distributor.