Primary Cervical Epithelial Cells
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ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
The basal medium for this primary cell is Cervical Epithelial Cell Basal Medium (ATCC PCS-480-032). To make the complete medium add the contents of Cervical Epithelial Cell Growth Kit (ATCC PCS-480-042) to the basal medium.
Every alternate day, remove medium and feed 5 mL of supplemented medium. However, when cultures reach 50% (or greater) confluence, remove medium and feed with 5 to 8 mL of supplemented medium daily.
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Woodworth CD, et al. Interleukin 1 alpha and tumor necrosis factor alpha stimulate autocrine amphiregulin expression and proliferation of human papillomavirus-immortalized and carcinoma-derived cervical epithelial cells. PNAS 92(7): 2840-844, 1995. PubMed: 7708734
Pecoraro G, et al. Differential effects of human papillomavirus type 6, 16, and 18 DNAs on immortalization and transformation of human cervical epithelial cells PNAS 86(2): 563-567, 1989. PubMed: 2463631
Ye F, et al. Stem-cell-abundant proteins Nanog, Nucleostemin and Musashi1 are highly expressed in malignant cervical epithelial cells. BMC Cancer 8: 108, 2008. PubMed: 18419830