ATCC ATCC Logo 0

Metagenomic Control Material for Pathogen Detection

MSA-4000

ATCC® Microbiome Standards are mock microbial communities that mimic mixed metagenomic samples. This product comprises genomic DNA prepared from fully sequenced, characterized, and authenticated ATCC Genuine Cultures, and was quantitated using Droplet Digital™ PCR to improve precision in relative abundance among strains within the community.
Product category
Bacteria
Components
0.1% Acinetobacter baumannii strain 5377 (ATCC 17978)
0.7% Enterococcus faecalis strain V583 (ATCC 700802)
1.4% Escherichia coli strain CFT073(ATCC 700928)
14.4% Klebsiella pneumoniae subsp. pneumoniae strain MGH78578 (ATCC 700721)
28.9% Neisseria meningitidis strain FAM18 (ATCC 700532)
0.3% Pseudomonas aeruginosa strain PAO1-LAC (ATCC 47085)
0.7% Staphylococcus aureus subsp. aureus (MRSA) strain FPR3757 (ATCC BAA-1556)
14.4% Staphylococcus aureus subsp. aureus (MSSA) strain TCH959 (ATCC BAA-1718)
2.9% Streptococcus agalacitae strain 2603 V/R (ATCC BAA-611)
28.9% Streptococcus pneumoniae strain Spain 23F-1 (ATCC 700669)
7.2% Streptococcus pyogenes strain SF370 (ATCC 700294)
Applications
Bioinformatics
Microbiome research
Next-generation sequencing
Product format
Frozen
Shipping information
Stored in Tris-HCl pH 8.5
Storage conditions
-20°C or colder
Buy Now
Price: $497.00 ea
Discounts are available for our fellow nonprofit organizations.

Generally ships within 1-3 business days

Documentation

Haven't found what you need?

Customize solution

We tailor our materials for your specific application.

Get application-specific materials

Biosafety Icon BSL 1

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Required Products

These products are vital for the proper use of this item and have been confirmed as effective in supporting functionality. If you use alternative products, the quality and effectiveness of the item may be affected.

Molecular Grade Water

60-2450

Price: $40.00 ea

Detailed product information

General

Specific applications
ATCC® Microbiome Standards enable the optimization of metagenomics workflows and microbiome research applications, providing reliable comparative data while improving assay consistency.

This product is ideal for pathogen detection, assay development, and as a daily run control for molecular diagnostics and microbiome applications.

For more information on the applications and use of this metagenomic control material, please review the publication "Assessing the Accuracy of Quantitative Molecular Microbial Profiling"

Characteristics

Genome sequenced strain
Yes
Comments
ATCC® Microbiome Standards are mock microbial communities that mimic mixed metagenomic samples. The LGC Metagenomic Control Material for Pathogen Detection was developed in partnership with the LGC Group, an international leader in genomics, measurement standards, and reference materials, as an ATCC Microbiome Standard representing a mock microbial community of bacterial species commonly observed in clinical infections, including antimicrobial-resistant organisms. The product comprises genomic DNA prepared from fully sequenced, characterized, and authenticated ATCC Genuine Cultures®, and was quantitated using Droplet Digital™ PCR to improve precision in relative abundance among strains within the community (refer to the Certificate of Analysis for more information). Each order for this product is provided with access to run 20 analyses on One Codex, the leading bioinformatics platform for microbial genomics and metagenomics.

Specification Range: 0.80 ng/µL to 1.33 ng/µL*
*DNA concentrations indicate ATCC manufacturing specifications and are provided as a reference only


LGC Standards Logo


© LGC Limited, 2017. All rights reserved.

Handling information

Handling notes
Aliquoting is highly recommended to avoid multiple freeze-­thaws, which can damage the DNA.

Quality control specifications

Volume
50 μL

History

Special collection
ATCC® Microbiome Standards

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.

Purchase of this product will include limited use access to the One Codex platform for metagenomic analysis of ATCC microbiome products. By purchasing this product, you consent and agree to allow ATCC to securely transfer sales and end users’ data related to this product to One Codex. Provision of One Codex access, storage, management of sequencing data, and the interpretation and accuracy of analytical results provided by One Codex are the responsibility of One Codex and not ATCC. End users should visit onecodex.com for their complementary metagenomic data analysis.


One Codex
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

References

Curated Citations

O'Sullivan DM, et al. Assessing the Accuracy of Quantitative Molecular Microbial Profiling. Int J Mol Sci 15(11): 21476-21491, 2014. PubMed: 25421243

Huggett JF, et al. Considerations for the development and application of control materials to improve metagenomic microbial community profiling. Accred Qual Assur 18(2): 77-83, 2013.

Frequently Asked Questions

Need assistance with this product? Contact our Technical Support team.

Telephone

Telephone

US and Puerto Rico
800-638-6597

Outside the US
+1-703-365-2700
hours

Hours of Operation

Monday-Friday
9:30am - 5:30pm
US Eastern Time