IDH1 mutant-U-87 Isogenic Cell Line
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Cells contain cytomegalovirus (CMV) DNA sequences
Cells contain SV40 sequences
ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
This is a glioma IDH1R132H mutant isogenic line derived from the parental U-87MG (ATCC HTB-14) cell line. The c.395G>A knock-in mutation encoding IDH1R132H protein expression was generated at ATCC by utilizing the CRISPR/Cas9 gene editing technology. This is a heterozygous mutation expressing the c.395G>A mutant allele. The IDH1R132H mutation in HTB-14IG has been validated at the genomic, transcript, and protein bio-functional levels.
The IDH1R132H mutation is among the most common seen in glioma and serves as an important diagnostic marker in various stages of disease in patients. This IDH1R132H mutant isogenic line HTB-14IG has been tested at ATCC for neomorphic functional activity displaying elevated levels of intra- and extracellular D-2HG above the parental U-87MG line. In addition, the HTB-14IG IDH1 mutant isogenic line displays histone hypermethylation compared to the parental line. This IDH1R132H isogenic cell model is a valuable in vitro cell-based tool for clinical diagnostics, elucidating mechanisms involved in cancer-associated differentiation, tumorigenesis and use in screening anti-cancer compounds for drug discovery and development.
Refer to the parental line U-87MG (ATCC HTB-14) for additional background information.
To ensure the highest level of viability, thaw the vial and initiate the culture as soon as possible upon receipt. If upon arrival, continued storage of the frozen culture is necessary, it should be stored in liquid nitrogen vapor phase and not at -70°C. Storage at -70°C will result in loss of viability.
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Avellaneda Matteo D, et al. Molecular mechanisms of isocitrate dehydrogenase 1 (IDH1) mutations identified in tumors: The role of size and hydrophobicity at residue 132 on catalytic efficiency. J Biol Chem 292(19): 7971-7983, 2017. PubMed: 28330869
Bralten LB, et al. IDH1 R132H decreases proliferation of glioma cell lines in vitro and in vivo. Ann Neurol 69(3):455-463, 2011. PubMed: 21446021
Rossetto M, et al. Metabolism of glioma and IDH1/IDH2 mutations. Rev Neurol 167(10): 699-703, 2011. PubMed: 21885076