hTERT-immortalized White Preadipocytes
CRL-4063 ™
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CRL-4063 ™
Fibroblast-like
ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.
ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
This newly created cell line can be used as an in vitro cell model for toxicity studies and the study of obesity and related diseases.
Near Diploid; Human Male Karyotype
The base medium for this cell line is DMEM:F12 Medium (ATCC 30-2006). To make the complete medium add the following components to the base medium:
To ensure the highest level of viability, thaw the vial and initiate the culture as soon as possible upon receipt. If upon arrival, continued storage of the frozen culture is necessary, it should be stored in liquid nitrogen vapor phase and not at -70°C. Storage at -70°C will result in loss of viability.
Complete culture medium + 8% DMSO (ATCC 4-X)
Marker Expression by flow cytometry: CD90+, CD166+, CD34-. Differentiation and positive Oil Red O Staining
ATCC
The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid. Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.
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For-profit organizations
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Cero C, et al. Standardized in vitro Models of Human Adipose Tissue Reveal Metabolic Flexibility in Brown Adipocyte Thermogenesis. Endocrinology 2023 Nov 7:bqad161. doi: 10.1210/endocr/bqad161. PubMed: 37944134
Xue R, et al. Clonal analyses and gene profiling identify genetic biomarkers of the thermogenic potential of human brown and white preadipocytes. Nat Med 21(7):760-768, 2015. PubMed: 26076036
Shinoda K, et al. Genetic and functional characterization of clonally derived adult human brown adipocytes. Nat Med 21(4):389-394, 2015. PubMed: 25774848
Markussen LM, et al. Characterization of immortalized human brown and white pre-adipocyte cell models from a single donor. PLoS ONE 12(9): e0185624, 2017. PubMed: 28957413