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Derived from CHO cells (see Urlaub et al.,1983; Urlaub et al.,1986) Cell line genetically modified.
A CHO-K1 DG44 cell line was stably transfected with a two-vector system expressing a model mouse-human chimeric IgG1 antibody. Single cell derived clones were isolated by limiting dilution and expanded. Agarabi CHO was subjected to further amplification by treatment with 2 µM methotrexate for up to 42 days and frozen. On thaw, Agarabi CHO was serially subcultured in the absence of methotrexate on a 3-4 day regime for ~2-3 weeks and cryopreserved.
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Velugula-Yella SR, et al. Impact of media and antifoam selection on monoclonal antibody production and quality using a high throughput micro‐bioreactor system. Biotechnol Prog 34(1): 262-270, 2018. PubMed: 29086492
Velugula-Yella SR, et al. Use of High-Throughput Automated Microbioreactor System for Production of Model IgG1 in CHO Cells. J Vis Exp 139 DOI: 10.3791/58231, 2018. PubMed: 30320757