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Cells contain SV40 sequences
ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
The transformed human microglial cells retain the properties of primary microglial cells. They represent homogeneous cell populations which can be grown indefinitely and might represent a convenient system for the biochemical analysis of the functions of microglial cells. As demonstrated in preliminary experiments, they can also support subsequent transfections which would allow study of the regulation of expression of various transfected genes in microglial cells.
To ensure the highest level of viability, thaw the vial and initiate the culture as soon as possible upon receipt. If upon arrival, continued storage of the frozen culture is necessary, it should be stored in liquid nitrogen vapor phase and not at -70°C. Storage at -70°C will result in loss of viability.
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Nakagawa Y, Chiba K. Diversity and plasticity of microglial cells in psychiatric and neurological disorders. Pharmacol Thera 154: 21–35, 2015. PubMed: 26129625
Janabi N, et al. Establishment of human microglial cell lines after transfection of primary cultures of embryonic microglial cells with the SV40 large T antigen. Neurosci Lett 195(2): 105-108, 1995. PubMed: 7478261
Li B, et al. NOX4 expression in human microglia leads to constitutive generation of reactive oxygen species and to constitutive IL-6 expression. J Innate Immun 1(6): 570–581, 2009. PubMed: 20375612
Jadhav VS, et al. HIV-1 Tat C modulates NOX2 and NOX4 expressions through miR-17 in a human microglial cell line. J Neurochem 131(6): 803-815, 2014. PubMed: 25146963
Dello Russo C, et al. The human microglial HMC3 cell line: where do we stand? A systematic literature review. J Neuroinflammation 15(1):259, 2018. PubMed: 30200996