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ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
Primary cells were isolated from healthy part of prostate cancer patient. The cell is immortalized by hTERT alone.
hTERT immortalized EP156T cell growth is inhibited by the presence of Normal Associated Fibroblast (NAFs, ATCC® CRL-3291™), while growth is promoted by the presence of Cancer Associated Fibroblast (CAFs, ATCC® CRL-3290™) using a transwell co-culture system.
Conclusion: hTERT immortalized EP156T cells provide a model system for the study of cancer cell progression and tumor microenvironment.
To ensure the highest level of viability, thaw the vial and initiate the culture as soon as possible upon receipt. If upon arrival, continued storage of the frozen culture is necessary, it should be stored in liquid nitrogen vapor phase and not at -70°C. Storage at -70°C will result in loss of viability.
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