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Cells produce the mouse xenotropic retrovirus Bxv-1
ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
Recently, it has been shown that VCaP prostate cancer cells produce the mouse xenotropic retrovirus Bxv-1, which was likely acquired by the cells during their xenotransplantation in mice.
ATCC® CRL-2876 ™ is a very slow growing cell line and can take up to 48 hours to attach post-thaw and after subcultures. It can routinely take a minimum of 2 weeks or more for cells to reach approximately 50% confluence with a dense mixture of adherent cells, floating clusters and moderate to heavy debris is always present. The cells may recover better in a T-25 flask compared to a T75 flask. Do not discard any floating cells that may be present during medium changes and subcultures. Instead, spin them down using gentle centrifugation and add them back to the adherent population. The cells attach in small tightly formed clusters and some single cells. As the cells attach and start to proliferate and spread, they will grow as flattened epithelial-like islands of tightly packed cells.
To insure the highest level of viability, thaw the vial and initiate the culture as soon as possible upon receipt. If upon arrival, continued storage of the frozen culture is necessary, it should be stored in liquid nitrogen vapor phase and not at -70°C. Storage at -70°C will result in loss of viability.
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Korenchuk S, et al. VCaP, a cell-based model system of human prostate cancer . In Vivo 15: 163-168, 2001. PubMed: 11317522
Knouf EC, et al. Multiple integrated copies and high-level production of the human retrovirus XMRV from 22Rv1 prostate carcinoma cells. J. Virol. 83: 7353-7356, 2009. PubMed: 19403664
Sfanos KS, et al. Identification of replication competent murine gamma retroviruses in commonly used prostate cancer cell lines. PLoS One 6:e20874, 2011.