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ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
Approximately 50% of LS513 cells express surface carcinoembryonic antigen (CEA).
MHC class II antigens (HLA-DR, DQ, and DP were not detected).
TGF beta-1 is inhibitory for proliferation of LS513 cells, whereas TGF beta-2 has no effect on the growth of these cells.
LS513 cells are 100-fold less sensitive to TGF beta-1 than the LS1034 (ATCC CRL-2158) cell line.
LS513 cells are multidrug resistant (MDR) and are tumorigenic in nude mice.
Colony forming efficiency was 30% in methylcellulose.
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Suardet L, et al. Responsiveness of three newly established human colorectal cancer cell lines to transforming growth factors beta 1 and beta 2. Cancer Res. 52: 3705-3712, 1992. PubMed: 1617643
Burmester JK, et al. Characterization of distinct functional domains of transforming growth factor beta. Proc. Natl. Acad. Sci. USA 90: 8628-8632, 1993. PubMed: 7690965
Li CY, et al. Potential role of WAF1/Cip1/p21 as a mediator of TGF-beta cytoinhibitory effect. J. Biol. Chem. 270: 4971-4974, 1995. PubMed: 7890601