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Cells contain papovavirus
ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
This is a polarized epithelial cell line which retains many differentiated characteristics of the terminal IMCD including inhibition of apical to basal sodium flux by amiloride and by atrial natriuretic peptide (ANP).
The cells possess an amiloride sensitive sodium channel as determined by Western blot analysis, and accumulate the major organic osmolytes (inositol, sorbitol, betaine and glycerophosphorylcholine) in response to hypertonic stress.
The cells secrete endothelin and form tubules and tight junctions.
mIMCD-3 cells are responsive to Hepatocyte Growth Factor (HGF), and are readily adaptable to growth in hypertonic medium supplemented with NaCl and urea up to 910 mosmol/kg H20. These extreme osmotic conditions exist in the renal medulla in vivo, but are known to be lethal to most other cells.
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Rauchman MI, et al. An osmotically tolerant inner medullary collecting duct cell line from an SV40 transgenic mouse. Am. J. Physiol. 265: F416-F424, 1993. PubMed: 8214101