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TALL-104

CRL-11386

Product category
Human cells
Organism
Homo sapiens, human
Cell type
T lymphoblast
Morphology
lymphoblast
Tissue
Peripheral blood
Disease
Acute lymphoblastic leukemia ALL
Applications
3D cell culture
Immune system disorder research
Immunology
Immuno-oncology
Product format
Frozen
Storage conditions
Vapor phase of liquid nitrogen
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Documentation

Biosafety Icon BSL 1

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Required Products

These products are vital for the proper use of this item and have been confirmed as effective in supporting functionality. If you use alternative products, the quality and effectiveness of the item may be affected.

RPMI-1640 Medium

30-2001

Price: $22.00 ea

Fetal Bovine Serum (FBS)

30-2020

Price: $645.00 ea

Dimethylsulfoxide (DMSO)

4-X

Price: $49.00 ea

Detailed product information

Characteristics

Growth properties
Suspension
Derivation
The TALL-104 line was established from the peripheral blood of a child in relapse with T-ALL.
Age
2 years
Gender
Male
Karyotype
46, XY; t(11;14)(p13;q11)
Antigen expression
CD2 +; CD3 +; CD7 +; CD8 +; CD56 +; CD4 -; CD16 -
Genes expressed
interferon gamma (IFN gamma); tumor necrosis factor alpha (TNF alpha); granulocyte monocyte colony stimulating factor (GM-CSF)
Expression markers
T cell antigen receptor alpha/beta (TCR), expressed; interleukin 2 (IL-2), expressed; interleukin 2 (IL-2); T cell antigen receptor alpha/beta (TCR)
Comments
The TALL-104 line was established from the peripheral blood of a child in relapse with T-ALL.
The cells are highly cytotoxic and are capable of tumor destruction in vivo and in vitro.
IL-2 is required from optimal growth and long term cultivation, but the cells can be grown for short periods without IL-2 (growth will be slower).
The cells are positive for the alpha/beta TCR and negative for gamma/delta TCR.
Technical information
ATCC Technical Services does not have technical information on patent deposits that are not produced or characterized by ATCC. Additional information can be found in the corresponding patent available from the patent holder or with the U.S. and/or international patent office.

Handling information

Unpacking and storage instructions
  1. Check all containers for leakage or breakage.
  2. Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below ­-130°C, preferably in liquid nitrogen vapor, until ready for use.
Complete medium
The base medium for this cell line is RPMI-1640 (ATCC 30-2001). To make the complete growth medium, add the following components to 400 mL of the base medium:
  • Fetal Bovine Serum (ATCC 30-2020) to a final concentration of 20%.
  • 1 vial IL-2 IS (10 µg), premium grade (Miltenyi cat# 130-097744*). Contents of vial can be rehydrated with 1 mL of culture medium and then added directly to the bottle of formulated medium.
*Note: The biological activity of cat# 130-097-744 is at least 5.0 x 106 units/mg but may be as high as 9.0 x 106 units/mg per Miltenyi. This may cause the final formulation of IL-2 to be greater than 100 units/mL
Note: Complete culture medium has a recommended shelf life of one month when being stored at 2-8°C.  Vendor does not provide stability information, but suggested material should be good for up to a month after formulation.
Temperature
37°C
Atmosphere
95% Air, 5% CO2
Handling procedure

To insure the highest level of viability, thaw the vial and initiate the culture as soon as possible upon receipt. If upon arrival, continued storage of the frozen culture is necessary, it should be stored in liquid nitrogen vapor phase and not at -70°C.  Storage at -70°C will result in loss of viability.

  1. Thaw the vial by gentle agitation in a 37°C water bath. To reduce the possibility of contamination, keep the O-ring and cap out of the water.  Thawing should be rapid (approximately 2 minutes).
  2. Remove the vial from the water bath as soon as the contents are thawed, and decontaminate by dipping in or spraying with 70% ethanol. All of the operations from this point on should be carried out under strict aseptic conditions.
  3. Transfer the vial contents to a centrifuge tube containing 9.0 mL complete culture medium and spin at approximately 125 x g for 5 to 7 minutes.
  4. Resuspend cell pellet with the recommended complete medium (see the specific batch information for the culture recommended dilution ratio) and dispense into an appropriate size culture flask.  It is important to avoid excessive alkalinity of the medium during recovery of the cells.  It is suggested that, prior to the addition of the vial contents, the culture vessel containing the complete growth medium be placed into the incubator for at least 15 minutes to allow the medium to reach its normal pH (7.0 to 7.6).
  5. Incubate the culture at 37°C in a suitable incubator.  A 5% CO2 in air atmosphere is recommended if using the medium described on this product.
Subculturing procedure
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at least 8 X 105 cells/mL.
Note: IL-2 rapidly loses its potency in medium, it is important that fresh IL-2 be used. Successful growth of this cell line is very dependent upon the quality of IL-2 used in the growth medium. ATCC recommends using the highest quality IL-2 available.
Interval: Maintain between 4 X 105 and 1 X 10cells/mL. 
Medium Renewal: Add medium as cell density increases
Reagents for cryopreservation
RPMI-1640 + 20% Gibco™ Knockout SR (Thermofisher Scientific cat# 10828010) + 10% DMSO. Freeze at a cell density of at least 15 x 106 cells/mL. Cell culture tested DMSO is available as ATCC 4-X.

Quality control specifications

Mycoplasma contamination
Not detected
STR profiling
Amelogenin: X,Y
CSF1PO: 10,11
D13S317: 9,12
D16S539: 12
D5S818: 12,13
D7S820: 7,13
THO1: 7,9
TPOX: 8,9
vWA: 14,18

History

Deposited as
Homo sapiens
Depositors
Wistar Institute
Patent depository
This material was deposited with the ATCC Patent Depository to fulfill U.S. or international patent requirements. This material may not have been produced or characterized by ATCC.  As an International Depository Authority (IDA) for patent deposits, ATCC is required to complete viability testing only at time of initial deposit of patent material. Patent deposits are made available on behalf of the Depositor when the pertinent U.S. or international patent is issued, but material may not be used to infringe the patent claims.
Patent number
5,272,082

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Disclosures
This material is cited in a US and/or international patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Depositor of the party to which the material was furnished.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

References

Curated Citations

Santoli D, et al. Cytotoxic T-ALL cell lines and uses therefor. US Patent 5,272,082 dated Dec 21 1993

O'Connor R, et al. Growth factor requirements of childhood acute T-lymphoblastic leukemia: correlation between presence of chromosomal abnormalities and ability to grow permanently in vitro. Blood 77: 1534-1545, 1991. PubMed: 1706955

Cesano A, et al. Homing and progression patterns of childhood acute lymphoblastic leukemias in severe combined immunodeficiency mice. Blood 77: 2463-2474, 1991. PubMed: 2039829

Frequently Asked Questions

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