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ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
This STAT1 knockout MDCK cell line was derived from the parental MDCK cell line (ATCC CCL-34) at ATCC using CRISPR-Cas9 gene editing technology. This cell line carries short nucleotide insertions and deletions in the fourth exon of the STAT1 gene. This cell line does not express STAT1 protein.
STAT1 (Signal Transducer and Activator of Transcription 1) is a transcription factor required for the interferon-based cellular anti-viral response. The MDCK.STAT1KO cell line ATCC CCL-34-VHG has been validated at the genomic, transcript, and protein bio-functional levels. It exhibits significant increased viral titer and enhanced virus production capability when compared to its parental cell line.
To ensure the highest level of viability, thaw the vial and initiate the culture as soon as possible upon receipt. If upon arrival, continued storage of the frozen culture is necessary, it should be stored in liquid nitrogen vapor phase and not at -70°C. Storage at -70°C will result in loss of viability.
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If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.
Horvath CM, Darnell JE Jr. The Antiviral State Induced by Alpha Interferon and Gamma Interferon Requires Transcriptionally Active Stat1 Protein. J Virol 70: 647-650, 1996. PubMed: 8523587
Meraz MA, et al. Targeted disruption of the Stat1 gene in mice reveals unexpected physiologic specificity in the JAK-STAT signaling pathway. Cell 84: 431-442, 1996. PubMed: 860859
Cedrone E, et al. Tissue-culture adapted Influenza virus strains. ATCC Connection volume 29 (2): 4-5 and 15, 2009.
Haass C, et al. Polarized sorting of beta-amyloid precursor protein and its proteolytic products in MDCK cells is regulated by two independent signals. J. Cell Biol. 128: 537-547, 1995. PubMed: 7860629
Gaush CR, et al. Characterization of an established line of canine kidney cells (MDCK). Proc. Soc. Exp. Biol. Med. 122: 931-935, 1966. PubMed: 5918973