Mumps virus (ATCC® VR-106)

Classification: Paramyxoviridae, Rubulavirus  /  Product Format: frozen 1 mL per vial

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Classification Paramyxoviridae, Rubulavirus
Deposited As Mumps
Agent Mumps virus
Strain Enders
Applications
This strain was used for preparation of skin test and viral V and S complement fixation antigens.
Respiratory research
Biosafety Level 2

Biosafety  classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen 1 mL per vial
Storage Conditions -70°C or colder
Comments This strain was used for preparation of skin test and viral V and S CF antigens. It is of low virulence for human volunteers. GenBank: GU980052.1
Effect on Host
CPE, cell rounding, syncytia formation, and sloughing
Recommended Host
SL-29 (ATCC® CRL-1590™)
Growth Conditions
Temperature: 36°C
Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 36°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 5-11 days
Effect on Host
CPE, cell rounding, syncytia formation, and sloughing
Name of Depositor JF Enders, Children's Medical Center, Boston, Massachusetts
Source Pooled saliva from patients, Massachusetts
References

Andrews CH. A short description of the Myxovirus group (Influenza and related viruses). Virology. 2: 176-184, 1955. PubMed: 13267985.

Enders JF, et al. Immunity in mumps: I. Experiments with monkeys (Macacus mulatta). The development of complement-fixing antibody following infection and experiments on immunization by means of inactivated virus and convalescent human serum. J. Exp. Med. 81: 93-117, 1945. PubMed: 19871448.

Henle G, et al. The reactivity of various human sera with mumps complement fixation antigens. J. Exp. Med. 1: 133-147, 1948. PubMed: 18871883.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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