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Fetal Bovine Serum, ES Cell Qualified (ATCC® SCRR-30-2020™)

Product Format frozen 500 mL
Storage Conditions Store fetal bovine serum at -70°C. If storage at -70°C is not possible, then store at -20°C. Do not store fetal bovine serum at temperatures above -20°C for any length of time. Avoid repeated freeze-thaws by dispensing and storing fetal bovine serum in aliquots. Fetal bovine serum is supplied in PETG plastic bottles.
Applications

This serum is tested for ability to support the growth and maintenance of embryonic stem cells in an undifferentiated state. Undifferentiated embryonic stem cells are determined by colony morphology and expression of three pluripotent markers. The results are compared to a control lot of serum. The serum is triple filtered through 0.1µM filters and each lot of fetal bovine serum is tested for sterility. Fetal bovine serum is manufactured from fetal bovine blood collected in USDA inspected abattoirs located in the United States.
Comments The results were compared with cells grown with a control lot of serum.
Biosafety Level N/A
Quality Control Specifications

Sterility tests are performed on each lot of fetal bovine serum using current USP and EP methods. Specific tests for contaminants are listed below in the Specification Certificate.   This serum is tested for ability to support the growth and maintenance of embryonic stem cells in an undifferentiated state.  Undifferentiated embryonic stem cells are determined by colony morphology and expression of three pluripotent markers.  The results are compared to a control lot of serum. The serum is triple filtered through 0.1 μM filters and each lot of fetal bovine serum is tested for sterility.

Origin

This fetal bovine serum is manufactured from fetal bovine blood collected in USDA-inspected abattoirs located in the United States.


Test*

Specification

Sterility Testing (Current USP and EP)

Bacteria and Fungi

No growth

Virus Testing (9 CFR 113.53)

Fluorescent antibody

Bluetongue

Bovine Adenovirus

Bovine Parvovirus

Bovine Respiratory Syncytial Virus

Bovine Diarrhea Virus

Rabies

Reovirus

Cytopathogenic Agents (e.g. IBR)

Hemadsorbing Agents (e.g., PI3)

Not detected

 

 

 

 

 

 

Mycoplasma Testing

Large volume, direct culture                       Hoechst DNA stain                   

Pass

Endotoxin (Limulus Amebocyte Lysate -Gel Clot)

≤ 10 EU/mL

Hemoglobin (Spectrophotometric)

Report result

Growth Promotion

Pass

ES Cell Testing

Pass

*Please consult the Certificate of Analysis for lot-specific test results.