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NCI-H1975 Purified Genomic DNA, Quantitative (EGFR Mutants), 3 µg (ATCC® CRL-5908DQ)

Focus: Oncology  / 

Permits and Restrictions

View Permits View Restrictions

Focus Area Oncology
Product Format frozen 3 µg, target concentration 100 ng/µL Quantitative Genomic DNA stored in 1X TE buffer
Verified By ATCC Cell Line Authentication Service
Sanger Sequencing
Storage Conditions -20°C or colder
Comments

DNA taken from NCI-H1975 (ATCC® CRL-5908™) Cell Line and quantitated

Cell Line Description:

The NCI-H1975 cell line is a human lung non-small cell carcinoma cell line

Spin down before use

Quantified for oncology biomarker EGFR p.T790M and EGFR p.L858R via NGS and ddPCR

Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Quality Control Specifications

DNA Quality Control Information*:

 

Test

Specification

Electrophoresis 

DNA - M.W.

≥48 kb (or higher than uppermost band of the high MW DNA ladder)

Electrophoresis 

RNA - Content

No visible RNA detected in the agarose gel

STR

Identical STR profile to cell line source

DNA Concentration

(PicoGreen® method)

Report Results

Purity (A260/A280)

Ratio 1.7 to 2.1

Total DNA amount 

(PicoGreen® method)

≥ 3 µg

Mutation allelic frequency

Report results: NGS (Coverage > 10,000X)*

Absolute/relative gene
copies/ng DNA 

Report results: ddPCR™ (Average of nine data points)*

Electrophoresis

DNA - Digestion

Verified by restriction enzyme digestion

*Please consult the Certificate of Analysis for lot-specific test results.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Restrictions

The line is available with the following restrictions:

  1. This cell line was deposited at the ATCC by Dr. Adi F. Gazdar and is provided for research purposes only. Neither the cell line nor products derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells are provided as service to the research community. They are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied.
  2. Any proposed commercial use of the these cells, or their products, must first be negotiated with the National Cancer Institute (NCI).  For further information, please contact NCI’s Technology Transfer Center at NCI_TTC_Contact@mail.nih.gov or by phone at (240)-276-5514.