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TF-1.CN5a.1 (ATCC® CRL-2512™)

Organism: Homo sapiens, human  /  Cell Type: erythroblast  /  Tissue: bone marrow  /  Disease: erythroleukemia

Permits and Restrictions

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Organism Homo sapiens, human
Tissue bone marrow
Cell Type erythroblast
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 2 Cells contain CMV viral sequences

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease erythroleukemia
Age 35 years
Gender male
Ethnicity Japanese
Applications
This cell line was derived from the TF-1 cell line (ATCC CRL-2003).
The cells can be used to access the potency and to study signal transduction of CNTF.
Storage Conditions liquid nitrogen vapor phase
Derivation
This cell line was derived from the TF-1 cell line (ATCC CRL-2003).
Following transfection, a line of G418 resistant cells was obtained and named TF-1.CN5a.
Clinical Data
male
Japanese
35 years
Receptor Expression
alpha subunit of human ciliary neurotrophic factor (CNTF) (receptor is not expressed by TF-1 cells)
Comments
The cells stably express the alpha subunit of human ciliary neurotrophic factor (CNTF) receptor and CNTF supports the short term proliferation of the cells. Suggested assay conditions are washing the cells with RPMI to remove GM-CSF, then incubation in RPMI 1640 95%, fetal bovine serum 5%, and picogram/ml concentrations of CNTF. The cells maintain responsiveness for at least 193 population doublings in full maintenance medium.
This cell line was derived from the TF-1 cell line (ATCC CRL-2003).
TF-1 cells were transfected, using Transfectam, with the gene for the alpha subunit of human ciliary neurotrophic factor (CNTF) receptor ligated to the pCR3.1 vector.
The vector contains cytomegalovirus (CMV) and SV40 viral sequences and the neomycin resistance gene.
Following transfection, a line of G418 resistant cells was obtained and named TF-1.CN5a.
TF-1.CN5a cells were grown, transiently, in the absence of GM-CSF and with 2 ng/ml CNTF to establish the TF-1.CN5a.1 cell line.
The cells can be used to access the potency and to study signal transduction of CNTF.
Complete Growth Medium RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate supplemented with 2 ng/ml GM-CSF and 0.4 mg/ml G-418, 90%; fetal bovine serum, 10%
Subculturing Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 x 105 viable cells/mL.  Maintain cell density at a cell concentration between 1 x 105 and 1 x 106 cells/mL.

Medium Renewal:  Every 2 to 3 days
Cryopreservation

Complete growth medium described above supplemented with 5% (v/v) DMSO.  Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
STR Profile
Amelogenin: X,Y
CSF1PO: 13
D13S317: 8,9
D16S539: 9,12
D5S818: 13
D7S820: 12
TH01: 7,9
TPOX: 8
vWA: 15,17
Name of Depositor CJ Robinson
Deposited As human
Year of Origin 2003
References

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Product Sheet
Product Sheet
Certificate of Analysis
Certificate of Analysis
SDS
SDS
References

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.