HH (ATCC® CRL-2105)

Organism: Homo sapiens, human  /  Cell Type: T lymphocyte  /  Tissue: peripheral blood  /  Disease: cutaneous T cell lymphoma

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Organism Homo sapiens, human
Tissue
peripheral blood
Cell Type T lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Disease cutaneous T cell lymphoma
Age 61 years adult
Gender male
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Derivation
The line was isolated from a patient who was negative for anti human T lymphotropic virus type I (HTLV-I) antibody, and no HTLV-I sequences were detected in the cultured cells by Southern analysis.
Clinical Data
61 years adult
The line was isolated from a patient who was negative for anti human T lymphotropic virus type I (HTLV-I) antibody, and no HTLV-I sequences were detected in the cultured cells by Southern analysis.
Caucasian
male
Antigen Expression
CD2; Homo sapiens, expressed
CD3; Homo sapiens, expressed
CD4; Homo sapiens, expressed
CD8; Homo sapiens; negative
CD25; Homo sapiens; negative
CD30; Homo sapiens, expressed
HLA-DR; Homo sapiens, expressed
Receptor Expression
interleukin 2 (IL-2) beta chain, expressed
HeLa Markers N
Comments
Although HH cells lack the alpha chain of the IL-2 receptor (IL-2R alpha), they do possess a functional p70 beta chain (IL-2R beta) of the IL-2 receptor complex.
A culture submitted to the ATCC in June of 1992 was found to be contaminated with mycoplasma, and was cured by treatment with Ciprofloxacin.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 x 105 cells/mL and maintain between 1 x 105 and 1 x 106 cells/mL.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
STR Profile
Amelogenin: X
CSF1PO: 11,12
D13S317: 11,12
D16S539: 12
D5S818: 11,13
D7S820: 10
THO1: 9
TPOX: 8,11
vWA: 15,16
Name of Depositor G Starkebaum
References

Starkebaum G, et al. Establishment of an IL-2 independent, human T-cell line possessing only the p70 IL-2 receptor. Int. J. Cancer 49: 246-253, 1991. PubMed: 1879969

HH is a mature T cell line derived in 1986 from peripheral blood of a patient with aggressive cutaneous T cell leukemia/lymphoma. The line was established from blood taken after extensive chemotherapy.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Starkebaum G, et al. Establishment of an IL-2 independent, human T-cell line possessing only the p70 IL-2 receptor. Int. J. Cancer 49: 246-253, 1991. PubMed: 1879969

HH is a mature T cell line derived in 1986 from peripheral blood of a patient with aggressive cutaneous T cell leukemia/lymphoma. The line was established from blood taken after extensive chemotherapy.