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WI-38 VA-13 subline 2RA (ATCC® CCL-75.1)

Organism: Homo sapiens, human  /  Cell Type: fibroblast SV40 transformed  /  Tissue: lung  / 

Permits and Restrictions

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Organism Homo sapiens, human
Tissue lung
Cell Type fibroblast SV40 transformed
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 2 [Cells contain polyomavirus DNA sequences]

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Age 3 months gestation
Gender female
Ethnicity Caucasian
Applications
transfection host
Storage Conditions liquid nitrogen vapor phase
Karyotype modal number = 76; range = 45 to 89.
Karyotype unstable within hyperdiploid stemline range 73-78. Ninety percent of the cells examined have 2 or 3 large chromosomes with subterminal centromeres and 1-6 microchromosomes. Secondary constrictions were observed in 59% of the cells, 21% had breaks.
Clinical Data
3 months gestation
Caucasian
female
Virus Susceptibility Human poliovirus 2
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing

Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:10 is recommended
Medium Renewal: Twice per week
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37.0°C
STR Profile
Amelogenin: X
CSF1PO: 10,12
D13S317: 11
D16S539: 11,12
D5S818: 10
D7S820: 9,11
TH01: 9.3
TPOX: 8
vWA: 19,20
Isoenzymes
G6PD, B
Name of Depositor AJ Girardi
Deposited As Homo sapiens
Year of Origin March 1964
References

Girardi AJ, et al. SV40 transformation of human diploid cells. A parallel study of viral and karyologic parameters. Ann. Med. Exp. Biol. Fenn. 44: 242-254, 1966. PubMed: 4290661

Jensen F, et al. Autologous and homologous implantation of human cells transformed in vitro by simian virus 40. J. Natl. Cancer Inst. 32: 917-937, 1964.

Wang S, Vos JM. A hybrid herpesvirus infectious vector based on epstein-barr virus and herpes simplex virus type 1 for gene transfer into human cells in vitro and in vivo. J. Virol. 70: 8422-8430, 1996. PubMed: 8970963

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Girardi AJ, et al. SV40 transformation of human diploid cells. A parallel study of viral and karyologic parameters. Ann. Med. Exp. Biol. Fenn. 44: 242-254, 1966. PubMed: 4290661

Jensen F, et al. Autologous and homologous implantation of human cells transformed in vitro by simian virus 40. J. Natl. Cancer Inst. 32: 917-937, 1964.

Wang S, Vos JM. A hybrid herpesvirus infectious vector based on epstein-barr virus and herpes simplex virus type 1 for gene transfer into human cells in vitro and in vivo. J. Virol. 70: 8422-8430, 1996. PubMed: 8970963