Staphylococcus aureus subsp. aureus Rosenbach

1. Just prior to inoculation place the frozen vial at room temperature to thaw. 
2. Transfer the entire contents of the vial to a tube of #3 broth (5 to 6 mL).
3. Use several drops of the cell suspension to inoculate a #3 agar slant and/or plate.  Additional #3 broth tubes may be inoculated by transferring 0.5 mL of the primary tube to each addition broth tube. 
4. Incubate the tubes and plate at 37°C for 24 hours.

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