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Lactobacillus plantarum (Orla-Jensen) Bergey et al.

8014-MINI-PACK

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ATCC® 8014-MINI-PACK™ consists of 6 ready-to-use vials of ATCC® 8014™ frozen in 200 µL of glycerol stock, eliminating the need to rehydrate and culture the strain prior to use. Each vial is provided with a 2-D barcode for easy storage and tracking, as well as peel-off labels for fast and reliable recordkeeping.
Product category
Bacteria
Strain designation
17-5 [BUCSAV 217, BUCSAV 449, Glaxo 664, ICPB 2080, NCDO 82, NCIB 6376, NCIB 8014, NCIB 8030]
Type strain
No
Genome sequenced strain
Yes
Applications
Food testing
Quality control
Organic acid production
Product format
Frozen
Shipping information
6 ready-to-use vials containing the strain in glycerol stock
Storage conditions
-80°C or colder
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Price: $267.00 EA
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Documentation

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information

General

Specific applications
ATCC® 8014-MINI-PACK™ consists of 6 ready-to-use vials of ATCC® 8014™ frozen in 200 µL of glycerol stock, eliminating the need to rehydrate and culture the strain prior to use. Each vial is provided with a 2-D barcode for easy storage and tracking, as well as peel-off labels for fast and reliable recordkeeping. Developed by the leaders in microbial cultivation and preservation, ATCC® Minis provide a convenient, ready-to-use solution for handling quality control strains. ATCC® Minis are authenticated and backed by ATCC polyphasic testing – ensuring the same consistent and reliable reference materials you’ve come to trust for ATCC Genuine Cultures®. It is easy to ensure the quality of your products with ATCC® Minis – just open, plate, and go!

Assay of amino acids
Assay of biotin
Assay of calcium pantothenate
Assay of niacinamide nicotinamide
Assay of nicotinic acid niacin
Assay of pantothenic acid
Assay of tryptophan L-tryptophan
Metabolizes mevalonic acid
Produces lactic acid lactate
Quality control strain
Produces lactic acid from coconut water
Food testing
Pharmaceutical and Personal Care
Bacteriophage host
Synthesis of ribitol teichoic acids
Energy-producing pathways
Preceptrol
No

Characteristics

Comments
Each NCIB culture from a different depositor
Accumulation of biotin
D-biotin conversion and metabolism
Biotin transport and accumulation
Formation of acetylmethylcarbinol
Effect of pantothenate derivatives on growth and coenzyme-A synthesis
Sulfur nutrition
Nitrate reduction
Effect of oleic acid on free biotin uptake
Ribitol-5-phosphate dehydrogenase
DNA base composition (45.1 moles % GC)

Handling information

Medium
Temperature
37°C
Atmosphere
95% Air, 5% CO2
Handling procedure
Frozen mini-cryovials packed in dry ice should either be thawed immediately for use or stored at or below
-70°C until the expiration date printed on the label. Storage at -20°C is acceptable for up to 1 year.
  1. Thaw the bacterial strain upright using gentle agitation in a 25°C to 30°C water bath. Thawing will be rapid; approximately 2-3 minutes or until all ice crystals have melted.
  2. Immediately after thawing, wipe down the mini-cryovial with 70% ethanol and aseptically transfer the entire contents to a 5-6 mL tube of #416 broth or directly inoculate a #416 plate and/or #416 agar slant.
  3. Discard the empty vial. Do not refreeze any unused portion as it will result in a loss of viability.
  4. Incubate at 37°C for 24 hours in an atmosphere of 5% CO2.
Handling notes
When used as a Bacteriophage host, best results are achieved by using a 24 hours culture.
Purified genomic DNA of this strain is available as ATCC® 8014D-5™.
Additional information on this culture is available on the ATCC® web site at www.atcc.org.

Quality control specifications

Verification method
Whole-genome Sequencing

History

Deposited as
Lactobacillus arabinosus Fred et al.
Depositors
E McCoy
Special collection
ATCC® Minis
Cross references
GenBank X62346 L.plantarum plasmid 8014-2 DNA (from Bc1I to HincII site).
GenBank AF189765 Lactobacillus plantarum alpha-galactosidase (melA) gene, complete cds.
GenBank U97139 Lactobacillus plantarum 16S/23S ribosomal RNA large intergenic spacer region, tRNA-Ile and tRNA-Ala genes, complete sequence.
GenBank X99978 Lactobacillus plantarum citrulline biosynthetic gene cluster (carAB operon and argC, J, B, D, F-ccl operon) and usg and dsg partial sequences.

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

Frequently Asked Questions

References

Curated Citations

AACC Niacin--microbiological method. St. Paul, MN, 1999

Arch. Biochem. 16: 357-360, 1948.

Costilow RN, Humphreys TW. Nitrate reduction by certain strains of Lactobacillus plantarum. Science 121: 168, 1955. PubMed: 13225760

Lichstein HC, Waller JR. Factors affecting the accumulation of biotin by Lactobacillus arabinosus. J. Bacteriol. 81: 65-69, 1961. PubMed: 13761907

AOAC International Amino acids in vitamin preparations. Gaithersburg, MD:AOAC International;AOAC "Official Methods of Analysis of the AOAC International" 960.47.

View All Curated Citations for this Product

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