ATCC ATCC Logo 0

Aliivibrio fischeri (Beijerinck) Urbanczyk et al.

7744

Download Genome Learn about our Enhanced Authentication Initiative
Type strain.  Formerly Vibrio fischerii. 
Product category
Bacteria
Product type
Preceptrol culture
Strain designation
[NCMB 1281]
Type strain
Yes
Genome sequenced strain
Yes
Product format
Freeze-dried
Storage conditions
2°C to 8°C
Buy Now
Price: $67.00 EA
Discounts are available for our fellow nonprofit organizations.

Generally ships within 1-3 business days

Documentation

Haven't found what you need?

Customize solution

We tailor our materials for your specific application.

Get application-specific materials

Biosafety Icon BSL 1

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information

General

Preceptrol
Yes

Characteristics

Comments
Activation and inhibition of luciferase synthesis

Handling information

Medium
Temperature
26°C
Atmosphere
Aerobic
Handling procedure
  1. Open vial according to enclosed instructions or visit www.atcc.org for instructions.
  2. Rehydrate the entire pellet with approximately 0.5 mL of #101 broth. Aseptically transfer the entire contents to a 5-6 mL tube of #101 broth. Additional test tubes can be inoculated by transferring 0.5 mL of the primary broth tube to these secondary tubes.
  3. Use several drops of the primary broth tube to inoculate a #101 plate and/or #101 agar slant.
  4. Incubate at 26°C for 24-72 hours.
Handling notes

Vibrio fischeri (Beijerinck 1889) Lehmann and Neumann 1896 (Approved Lists 1980) and Photobacterium fischeri (Beijerinck 1889) Reichelt and Baumann 1973 (Approved Lists 1980) have the same type strain and therefore are homotypic synonyms (Rules 24a and 24b of the International Code of Nomenclature of Bacteria and Viruses).

 

This strain will grow on Marine Broth 2216 (BD 279110) and Marine Agar 2216 (BD 212185), but Photobacterium Medium is recommended for demonstrating luminescence. To check for luminescence, inoculate a slant, leaving cap loose. Incubate at 26oC for 18 hours. Then, hold in the dark for 10 minutes. If no luminescence is detected, reincubate and check again after 36, 48, and 72 hours. When maximum luminescence is obtained, stopper tightly.

This strain needs to establish good growth in broth culture before additional transfers are made. Additional incubation may be required for growth on solid media.
 

Additional information on this culture is available on the ATCC® web site at www.atcc.org.

Quality control specifications

Verification method
Whole-genome Sequencing

History

Deposited as
Achromobacter fischeri (Beijerinck) Bergey et al.
Depositors
FH Johnson
Cross references
GenBank X70289 V.fischeri luxG gene and orfV.
GenBank X02253 Vibrio fischeri (ATCC 7744) 5S rRNA.
GenBank X74702 V.fisheri (ATCC 7744T) gene for 16S ribosomal RNA.
GenBank AB039838 Vibrio fischeri fldA gene for flavodoxin 1, complete cds.
GenBank D17743 Vibrio fischeri gene for NAD(P)H-flavin oxidoreductase, complete cds.
GenBank AF023157 Vibrio fischeri cytotoxic necrotizing factor 1 (cnf1) gene, partial cds.
GenBank M25752 V.fischeri luxR transcriptional activator gene, clone PVFB1, complete cds.
GenBank E06567 A genomic DNA encoding an enzyme with flavin nucleotide and nitro-group reducing activities.
GenBank E06568 A genomic DNA encoding an enzyme with flavin nucleotide and nitro-group reducing activities.
GenBank E06569 A genomic DNA encoding an enzyme with flavin nucleotide and nitro-group reducing activities.
GenBank M19039 V.fischeri regulatory protein (luxR), autoinducer (luxI) genes, complete cds, and luxC gene, 5' end.
GenBank AB039839 Vibrio fischeri xerD, fldB genes for site-specific recombinase, flavodoxin 2, partial and complete cds.
GenBank AB039955 Vibrio fischeri gene for cobalamin-dependent methionine synthase, Na+/H+ antiporter and aspartokinase III, partial and complete cds.

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

References

Curated Citations

Hendrie MS, et al. The identification, taxonomy and classification of luminous bacteria. J. Gen. Microbiol. 64: 151-169, 1970.

Hendrie MS, et al. Proposal that Vibrio marinus (Russell 1891) Ford 1927 be amalgamated with Vibrio fischeri (Beijerinck 1889) Lehmann and Neumann 1896. Int. J. Syst. Bacteriol. 21: 217-221, 1971.

Eberhard A. Inhibition and activation of bacterial luciferase synthesis. J. Bacteriol. 109: 1101-1105, 1972. PubMed: 5011244

Sitnikov DM, et al. Control of cell division in Escherichia coli: regulation of transcription of ftsQA involves both rpoS and SdiA-mediated autoinduction. Proc. Natl. Acad. Sci. USA 93: 336-341, 1996. PubMed: 8552633

Skerman VB, et al. Approved lists of bacterial names. Int J Syst Bacteriol 30: 225-420, 1980.

Frequently Asked Questions

Need assistance with this product? Contact our Technical Support team.

Telephone

Telephone

US and Puerto Rico
800-638-6597

Outside the US
+1-703-365-2700
hours

Hours of Operation

Monday-Friday
9:30am - 5:30pm
US Eastern Time