Virgibacillus marismortui (Arahal et al.) Heyrman et al.
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2. From a single tube of #2101 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette and use to rehydrate the pellet.
3. Aseptically transfer the rehydrated pellet back into the broth tube. Mix well.
4. Use several drops of this suspension to inoculate an additional broth tube, a #2101 agar slant and/or a plate.
5. Incubate the tubes and plate at 37°C for 24 to 48 hours.
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Arahal DR, et al. Bacillus marismortui sp. nov., a new moderately halophilic species from the Dead Sea. Int. J. Syst. Bacteriol. 49: 521-530, 1999. PubMed: 10319473
Arahal DR, et al. Reclassification of Bacillus marismortui as Salibacillus marismortui comb. nov.. Int. J. Syst. Evol. Microbiol. 50: 1501-1503, 2000. PubMed: 10939656