Lactobacillus plantarum (Orla-Jensen) Bergey et al.
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2. From a single tube of #416 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette and use to rehydrate the entire pellet.
3. Aseptically transfer the rehydrated pellet back into the broth tube. Mix well
4. Use several drops of this suspension to inoculate a second tube of broth, a slant and/or a plate.
5. Incubate tubes and plate at 30oC in a 5% CO2 atmosphere for 24-48 hours. Loosen screw caps of all test tubes during the incubation period.
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Jewell B, Collins-Thompson DL. Characterization of chloramphenicol resistance in Lactobacillus plantarum CaTC2. Curr. Microbiol. 19: 343-346, 1989.
Sinha RP. Plasmid instability in Lactobacillus plantarum strain caTC2. Curr. Microbiol. 25: 219-223, 1992.