Micromonospora matsumotoense (Asano et al.) Lee et al.
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2. Using a single tube of #1877 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a #196 agar slant and/or plate.
5. Incubate the tubes and plate at 26oC for 5 to 7 days.
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Asano K, et al. Catellatospora matsumotoense sp. nov. and C. tsunoense sp. nov., actinomycetes found in woodland soils. Int. J. Syst. Bacteriol. 39: 309-313, 1989.
Lee SD, et al. A phylogenetic analysis of the genus Catellatospora based on 16S ribosomal DNA sequences, including transfer of Catellatospora matsumotoense to the genus Micromonospora as Micromonospora matsumotoense comb. nov.. FEMS Microbiol. Lett. 178: 349-354, 1999. PubMed: 10499285