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Mycoplasma synoviae Olson et al. emend. Jordan et al.


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Mycoplasma synoviae strain WVU 1853 [NCTC 10124] is a whole-genome sequenced type strain that was isolated from the hock joint of a chicken. This bacterial culture has applications in agricultural research, media testing, quality control, and pharmaceutical testing.
Product category
Strain designation
WVU 1853 [NCTC 10124]
Type strain
Genome sequenced strain
Isolation source
Hock joint of chicken
Agricultural research
Media testing
Quality control
Pharmaceutical testing
Product format
Storage conditions
2°C to 8°C
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ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information


Specific applications
Control strain
Media testing
Pharmaceutical and Personal Care
Recommended as a test microorganism for EP 2.6.7 Mycoplasmas
Recommended as a test microorganism for USP <63> Mycoplasma Tests

Handling information

Broth: Aerobic
Plates: 10% CO2
Handling procedure
  1. Follow instructions as suggested for the culturing of Mollicutes: 


    1. Using a Pasteur or 1.0 mL pipette withdraw approximately 0.5 to 1.0 mL from a tube containing 2.5 mL. Rehydrate the entire pellet.
    2. Aseptically transfer the entire aliquot back into the tube. Mix well.
    3. Perform 1:10 serial dilutions in broth. Many strains will die out rapidly once acid or alkaline conditions are reached. It is recommended to prepare several dilutions from the initial tube to keep the culture in various stages of growth and to prevent inhibition of growth by the cryoprotectant used in the freeze-drying process.
    4. Use an uninoculated tube of broth to serve as a control.
    5. Plates may be inoculated to check colony morphology. Spot 0.1 mL of each dilution on the surface of a plate to determine the number of colony-forming units. Not all strains do well on solid medium.
    6. Incubate all tubes at 37°C aerobically for 48 to 72 hours. Incubate plates at 37°C in 10% CO2 for 7 to 10 days.
    7. This strain will show light turbidity in broth. ATCC medium #2764 does not contain a pH indicator, therefore, there will not be a significant change in color. Colonies are pinpoint, clear and glistening.
  1. Subsequent fresh transfers may grow more rapidly than the original rehydrated culture. 
Handling notes
Additional information on this culture is available on the ATCC® web site at

Quality control specifications

Verification method
Whole-genome Sequencing


Deposited as
Mycoplasma synoviae Olson et al. emend. Jordan et al.
RG Wittler
Chain of custody
ATCC <-- RG Wittler <-- N Olson WVU 1853
Type of isolate

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.


This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.



Curated Citations

Edward DG, Freundt EA. Type strains of species of the order Mycoplasmatales, including designation of neotypes for Mycoplasma mycoides subsp. mycoides, Mycoplasma agalactiae subsp. agalactiae, and Mycoplasma arthritidis. Int. J. Syst. Bacteriol. 23: 55-61, 1973.

Olson NO, et al. Control of infectious synovitis. 12. Preparation of an agglutination test antigen. Avian Dis. 7: 310-317, 1963. PubMed: 5896498

Olson NO, et al. . Avian Dis. 8: 209-214, 1964.

Jordan FT, et al. Characterization and taxonomic description of five mycoplasma serovans (serotypes) of avian origin and their elevation to species rank and further evaluation of the taxonomic status of Mycoplasma synoviae. Int. J. Syst. Bacteriol. 32: 108-115, 1982.

British Pharmacopoeia Commission Tests for microbial contamination. London, UK:British Pharmacopoeia Commission;British Pharmacopoeia Appendix XVI B, 2003

View All Curated Citations for this Product

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