Escherichia coli (Migula) Castellani and Chalmers (ATCC® 9637)

Strain Designations: [397E, CCM 2024, DSM 1116, IFO 13500, NCIB 8666, NRRL B-766, W]  /  Type Strain: no  /  Biosafety Level: 1

Permits And Restrictions

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Classification Enterobacteriaceae, Escherichia
Strain Designations [397E, CCM 2024, DSM 1116, IFO 13500, NCIB 8666, NRRL B-766, W]
Assay of colistin colimycin
Control strain
Produces 6-aminopenicillanic acid
Produces 6-aminopenicillanic acid amides
Produces cephalosporins
Susceptibility testing
Deacylation of benzyl- and phenoxymethyl penicillin tetrazoles
Produces cephalosporins by conversion
Pharmaceutical and Personal Care
Type Strain no
Biosafety Level 1
Product Format freeze-dried
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Preceptrol® yes
Type Strain no
Diaminopimelate decarboxylase activity
Medium ATCC® Medium 3: Nutrient agar or nutrient broth
Growth Conditions
Temperature: 37°C
Atmosphere: Aerobic
Name of Depositor SA Waksman

Huang HT, English AR. Production of 6-aminopenicillanic acid. US Patent 3,239,427 dated Mar 8 1966

Takahashi T, et al. Method for the production of cephalosporins. US Patent 3,945,888 dated Mar 23 1976

Hamsher JJ. Enzymatic deacylation of benzyl- and phenoxymethyl penicillin tetrazoles. US Patent 3,905,868 dated Sep 16 1975

Huang HT. 6-Aminopenicillanic acid amide production. US Patent 3,088,880 dated May 7 1963

Science 114: 459, 1951.

British Pharmacopoeia Commission Biological assay of antibiotics. London, UK:British Pharmacopoeia Commission;British Pharmacopoeia Appendix XIV A, 2003

European Pharmacopoeia Commission Microbiological assay of antibiotics. Strasbourg, France:European Pharmacopoeia Commission;European Pharmacopoeia EP 2.7.2, 1997

White PJ. The regulation of diaminopimelate decarboxylase activity in Escherichia coli strain w. J. Gen. Microbiol. 96: 51-62, 1976. PubMed: 789815

8310 B: Growth inhibition test with freshwater ciliate Colpidium campylum. Washington, DC:American Public Health Association;Standard Methods for the Examination of Water and Wastewater, 2005 APHA8310 - Ciliated Protozoa;.

Medical microbiology; methods for the determination of susceptibility of pathogens (except mycobacteria) to antimicrobial agents: carrier of agents for agar diffusion test. Berlin, Germany:Deutsches Institut fur Normung;DIN DIN 58940-2.

Methods for the determination of susceptibility of pathogenic bacteria (without mycobacteria) to chemotherapeutic agents of discs for agar diffusion test example for getting the standard curve. Berlin, Germany:Deutsches Institut fur Normung;DIN DIN 58940-2 Beiblatt 1.

Medical microbiology - Susceptibility testing of pathogens to antimicrobial agents - Part 2: Active substance carriers for the agar diffusion test; Carrier loads and values required for drawing a standard curve. Berlin, Germany: Deutsches Institut fur Normung; DIN 58940-2 Beiblatt 2: 2002

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation