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SK-BR-3 Purified Genomic DNA, Quantitative (TP53 Mutant), 3 µg (ATCC® HTB-30DQ)

Focus: Oncology  / 

Permits and Restrictions

View Permits

Focus Area Oncology
Product Format frozen 3 µg, target concentration 100 ng/µL Quantitative Genomic DNA
stored in 1X TE buffer
Storage Conditions -20°C or colder

DNA taken from SK-BR-3 (ATCC® HTB-30™) Cell Line and quantitated

Cell Line Description:

The SK-BR-3 cell line is a human breast adenocarcinoma cell line

Spin down before use

Quantitated for biomarker TP53 pR175H via NGS and ddPCR

Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Quality Control Specifications

DNA Quality Control Information*:





DNA - M.W.

≥48 kb (or higher than uppermost band of the high MW DNA ladder)


RNA - Content

No visible RNA detected in the agarose gel


Identical STR profile to cell line source

DNA Concentration

(PicoGreen® method)

Report Results

Purity (A260/A280)

Ratio 1.7 to 2.1

Total DNA amount 

(PicoGreen® method)

≥ 3 µg

Mutation allelic frequency

Report results: NGS (Coverage > 10,000X)*

Absolute/relative gene
copies/ng DNA 

Report results: ddPCR™ (Average of nine data points)*


DNA - Digestion

Verified by restriction enzyme digestion

*Please consult the Certificate of Analysis for lot-specific test results.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation