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HCT-15 Purified Genomic DNA, Quantitative (KRAS Mutant), 3 µg (ATCC® CCL-225DQ)

Focus: Oncology  / 

Permits and Restrictions

View Permits

Focus Area Oncology
Product Format frozen 3 µg, target concentration 100 ng/µL Quantitative Genomic DNA
stored in 1X TE buffer
Verified By ATCC Cell Line Authentication Service
Sanger Sequencing
Storage Conditions -20°C or colder
Comments

DNA taken from HCT-15 (ATCC® CCL-225™) Cell Line and quantitated

Cell Line Description:

The HCT-15 cell line is a colon adenocarcinoma cell line

Spin down before use

Quantified for oncology biomarker KRAS p.G13D via NGS and ddPCR

Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Quality Control Specifications

DNA Quality Control Information*:

 

Test

Specification

Electrophoresis 

DNA - M.W.

≥48 kb (or higher than uppermost band of the high MW DNA ladder)

Electrophoresis 

RNA - Content

No visible RNA detected in the agarose gel

STR

Identical STR profile to cell line source

DNA Concentration

(PicoGreen® method)

Report Results

Purity (A260/A280)

Ratio 1.7 to 2.1

Total DNA amount 

(PicoGreen® method)

≥ 3 µg

Mutation allelic frequency

Report results: NGS (Coverage > 10,000X)*

Absolute/relative gene
copies/ng DNA 

Report results: ddPCR™ (Average of nine data points)*

Electrophoresis

DNA - Digestion

Verified by restriction enzyme digestion

*Please consult the Certificate of Analysis for lot-specific test results.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation