FBHE (ATCC® CRL-1395)

Organism: Bos taurus, cow  /  Cell Type: endothelial  / 

Organism Bos taurus, cow
Cell Type endothelial
Product Format frozen
Morphology endothelial
Culture Properties adherent
Biosafety Level 2 Cells contain Bovine Viral Diarrhea Virus (BVDV)
Age fetus
Applications
The line is positive for bovine viral diarrhea virus (BVD).
Derivation
The FBHE cell line absolutely requires fibroblast growth factor (FGF) for survival, which can be supplied in the form of purified FGF or Bovine Pituitary Extract (BPE).
Comments
The FBHE cell line absolutely requires fibroblast growth factor (FGF) for survival, which can be supplied in the form of purified FGF or Bovine Pituitary Extract (BPE).
The line is positive for bovine viral diarrhea virus (BVD).
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:5 is recommended
Medium Renewal: Twice per week
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Subculture every 5 to 7 days , before 90% confluency.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Name of Depositor DJ Gospodarowicz
References

Bolin SR, et al. Survey of cell lines in the American Type Culture Collection for bovine viral diarrhea virus. J. Virol. Methods 48: 211-221, 1994. PubMed: 7989438

Gospodarowicz D, et al. Clonal growth of bovine vascular endothelial cells: fibroblast growth factor as a survival agent. Proc. Natl. Acad. Sci. USA 73: 4120-4124, 1976. PubMed: 1069301

Basic Documentation
References

Bolin SR, et al. Survey of cell lines in the American Type Culture Collection for bovine viral diarrhea virus. J. Virol. Methods 48: 211-221, 1994. PubMed: 7989438

Gospodarowicz D, et al. Clonal growth of bovine vascular endothelial cells: fibroblast growth factor as a survival agent. Proc. Natl. Acad. Sci. USA 73: 4120-4124, 1976. PubMed: 1069301