YB2/0 [YB2/3HL.P2.G11.16Ag.20] (ATCC® CRL-1662™) Organism: Rattus norvegicus, rat / Cell Type:: hybridoma: B lymphoblast / Tissue: spleen / General Information Characteristics Culture Method History Documentation Print Email Share Share this product Facebook Twitter Google+ Organism Rattus norvegicus, rat Tissue spleen Cell Type hybridoma: B lymphoblast Product Format frozen Morphology lymphoblast Culture Properties suspension Biosafety Level 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. Applications The cells are resistant to 8-azaguanine, are HAT sensitive and can be used as a fusion partner for rat B cells to make rat - rat hybridomas. Derivation This line was produced by fusing Y3/Ag1.2.3 (ATCC CRL-1631) cells with spleen cells from an AO strain rat. Comments Tested and found negative for ectromelia virus (mousepox). The cells do not secrete either heavy or light immunoglobulin chains. Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Subculturing Cultures are maintained by addition or replacement of fresh medium. Start new cultures at 1 X 105 viable cells/mL and maintain at or below 1 X 106 cells/mL. Medium Renewal: Every 2 to 3 days Cryopreservation Complete growth medium 95%; DMSO 5% (v/v). Cell culture tested DMSO is available as ATCC® Catalog No. 4-X. Name of Depositor C Milstein References Kilmartin JV, et al. Rat monoclonal antitubulin antibodies derived by using a new nonsecreting rat cell line. J. Cell Biol. 93: 576-582, 1982. PubMed: 6811596 . . Methods Enzymol. 73B: 1-46, 1981. Basic Documentation Product Sheet Certificate of Analysis SDS References Kilmartin JV, et al. Rat monoclonal antitubulin antibodies derived by using a new nonsecreting rat cell line. J. Cell Biol. 93: 576-582, 1982. PubMed: 6811596 . . Methods Enzymol. 73B: 1-46, 1981.