RM-2 (ATCC® CRL-3311)

Organism: Mus musculus, mouse  /  Tissue: Mouse prostate: Ras+Myc-induced prostate cancer that developed from a urogenital sinus mouse prostate reconstitution.  /  Disease: prostate cancer

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Organism Mus musculus, mouse
Tissue Mouse prostate: Ras+Myc-induced prostate cancer that developed from a urogenital sinus mouse prostate reconstitution.
Product Format frozen 1.0 mL
Morphology epithelial-like
Culture Properties adherent
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease prostate cancer
Age 17 days
Gender male
Strain C57BL/6
Applications A novel model system to study prostate cancer in vitro. This system includes ras+/myc- transformed clonal prostate cancer cell lines
Storage Conditions liquid nitrogen vapor phase
Images
Comments 17 day-old urogenital sinus cells of C57BL/6 embryos were infected with Zipras/myc9 retrovirus and grafted under the renal capsule of isogenic adult male mice.
Complete Growth Medium

The base medium for this cell line is DMEM: F12 (ATCC 30-2002). To make the complete medium add the following components to 500 mL of the base medium:

  • 56 mL fetal bovine serum (FBS; ATCC 30-2020)
Subculturing
Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Corning® T-75 flasks (catalog #430641) are recommended for subculturing this product.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with DPBS (ATCC catalog # 30-2200) to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
    Cultures can be established between 2.0 x 104 and 4.0 x 104 viable cells/cm2.
  6. Incubate cultures at 37°C.
Interval: Maintain cultures at a cell concentration between 1.0 X 104 and 3.5 X 105 cell/cm2.
Subcultivation Ratio: A subcultivation ratio of 1:8 to 1:10 is recommended
Medium Renewal: 2 to 3 times per week
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
Cells per Vial Approximately 1.5 to 2.5 x 106 cells
Volume 1.0 mL
Sterility Tests Bacteria and yeast: No growth
Mycoplasma: No growth
Viability ≥ 50%
Name of Depositor T Thompson, Genitourinary Medical Oncology, Baylor College of Medicine, Houston, TX
Year of Origin 1989
References

Thompson TC, et al. Multistage carcinogenesis induced by ras and myc oncogenes in a reconstituted organ. Cell 56(6): 917-930, 1989. PubMed: 2538247

Baley PA, et al. Progression to androgen insensitivity in a novel in vitro mouse model for prostate cancer. J Steroid Biochem Mol Biol 52(5): 403-413, 1995. PubMed: 7538321

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • For-profit customers must obtain a research use license agreement from the Contributor prior to shipment.
  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation
Restrictions

This material’s use is governed by the MTA and the following restrictions: (1) Prior to purchase, for-profit entities must obtain a research use license from Baylor College of Medicine and (2) when a Commercial Use is contemplated, for-profit and non-profit entities must obtain a commercial use license from Baylor College of Medicine before this item can be shipped. Contact Baylor Licensing Group, by phone: (713) 798-6821 or email: blg@bcm.tmc.edu.

References

Thompson TC, et al. Multistage carcinogenesis induced by ras and myc oncogenes in a reconstituted organ. Cell 56(6): 917-930, 1989. PubMed: 2538247

Baley PA, et al. Progression to androgen insensitivity in a novel in vitro mouse model for prostate cancer. J Steroid Biochem Mol Biol 52(5): 403-413, 1995. PubMed: 7538321