Ku80-/- is a cell line deficient in Ku80. When paired with its control Ku80+/+, it can be used in cancer drug discovery and to investigate the role of DNA double-strand break repair in chromosomal aberrations.
Together, Ku790 and Ku80 make up the Ku heterodimer, which binds DNA double-strand break ends and is required for the non-homologous end joining(NHEJ) pathway of DNA repair. It is also required for V(D)J recombination, which utilizes the NHEJ pathway to promote antigen diversity in the mammalian immune system.
These cells were immortalized by SV40