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cGMP Vero (ATCC® CCL-81.4)

Organism: Cercopithecus aethiops  /  Tissue: kidney  /  Disease: normal

Permits and Restrictions

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Organism Cercopithecus aethiops
Tissue kidney
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease normal
Age adult
Applications cGMP cells may be used for the production of viruses and proteins. These cells may also be employed in quality control applications.
Storage Conditions liquid nitrogen vapor phase
Karyotype Historically, this is a cell line with the hypodiploid chromosome count. The modal chromosome number was 58 occurring in 66% of cells. In most cells, over 50% of the chromosomes in each cell complement belonged to structurally altered marker chromosomes. Normal A3, A4, B4, and B5 were absent; B2, B3 and B7 were occasionally paired; and B9, C1 and C5 were mostly paired. The rate of cells with higher ploidies was 1.7%. Other chromosomes were mostly present in single copy.
Derivation
Historically, the Vero cell line was initiated from the kidney of a normal adult African green monkey on March 27, 1962, by Y. Yasumura and Y. Kawakita at the Chiba University in Chiba, Japan. 
Comments

Manufactured and distributed per cGMP regulations, cGMPs provide for systems that assure proper design, monitoring, and control of manufacturing processes and facilities.

Manufactured for ATCC by Charles River Laboratories, Malvern, PA.

For historical references refer to CCL-81.

Complete Growth Medium

These cells are grown in Eagle's Minimal Essential Medium (ATCC 30-2003), supplemented with:

  • 10% fetal bovine serum (FBS; ATCC 30-2020)
Subculturing
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes. Corning® T-75 flasks (catalog #430641) are recommended for subculturing this product.
    1. Remove and discard culture medium.
    2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
    3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
      Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
    4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
    5. Add appropriate aliquots of the cell suspension to new culture vessels.
    6. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
Sterility Tests

Negative

Functional Tests Bacteriostasis and fungistasis - no inhibition
Name of Depositor W Hann, JS Rhim for the parental cell line
Passage History
The parental cell line was brought to the Laboratory of Tropical Virology, National Institute of Allergy and Infectious Diseases, National Institutes of Health in the 93rd passage from Chiba University by B. Simizu on June 15, 1964.
Year of Origin
Parental cell line: 1962
cGMP bank: 2006
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • For-profit customers must obtain a research use license agreement from the Contributor prior to shipment.
  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Restrictions

The ATCC Material (and any Modifications, Unmodified Derivatives and/or Progeny thereof) may not be used (1) for commercial use by any customer, or (2) by for-profit customers for any purpose, without a license from ATCC.  For instructions on how to obtain a license, please contact licensing@atcc.org or complete and submit a licensing inquiry form by clicking this link: http://licensinginquiry.atcc.org/lilform.aspx.