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Escherichia coli (Migula) Castellani and Chalmers (ATCC® 9637)

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Strain Designations: [397E, CCM 2024, DSM 1116, IFO 13500, NCIB 8666, NRRL B-766, W]  /  Type Strain: no  /  Biosafety Level: 1

Permits and Restrictions

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Verified By Whole-genome Sequencing
Classification Enterobacteriaceae, Escherichia
Strain Designations [397E, CCM 2024, DSM 1116, IFO 13500, NCIB 8666, NRRL B-766, W]
Assay of colistin colimycin
Control strain
Produces 6-aminopenicillanic acid
Produces 6-aminopenicillanic acid amides
Produces cephalosporins
Susceptibility testing
Deacylation of benzyl- and phenoxymethyl penicillin tetrazoles
Produces cephalosporins by conversion
Pharmaceutical and Personal Care
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format freeze-dried
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Preceptrol® yes
Type Strain no
Diaminopimelate decarboxylase activity
Medium ATCC® Medium 3: Nutrient agar or nutrient broth
Growth Conditions
Temperature: 37°C
Atmosphere: Aerobic
Name of Depositor SA Waksman
U.S. Patent Number

Huang HT, English AR. Production of 6-aminopenicillanic acid. US Patent 3,239,427 dated Mar 8 1966

Takahashi T, et al. Method for the production of cephalosporins. US Patent 3,945,888 dated Mar 23 1976

Hamsher JJ. Enzymatic deacylation of benzyl- and phenoxymethyl penicillin tetrazoles. US Patent 3,905,868 dated Sep 16 1975

Huang HT. 6-Aminopenicillanic acid amide production. US Patent 3,088,880 dated May 7 1963

Science 114: 459, 1951.

British Pharmacopoeia Commission Biological assay of antibiotics. London, UK:British Pharmacopoeia Commission;British Pharmacopoeia Appendix XIV A, 2003

European Pharmacopoeia Commission Microbiological assay of antibiotics. Strasbourg, France:European Pharmacopoeia Commission;European Pharmacopoeia EP 2.7.2, 1997

White PJ. The regulation of diaminopimelate decarboxylase activity in Escherichia coli strain w. J. Gen. Microbiol. 96: 51-62, 1976. PubMed: 789815

8310 B: Growth inhibition test with freshwater ciliate Colpidium campylum. Washington, DC:American Public Health Association;Standard Methods for the Examination of Water and Wastewater, 2005 APHA8310 - Ciliated Protozoa;.

Medical microbiology; methods for the determination of susceptibility of pathogens (except mycobacteria) to antimicrobial agents: carrier of agents for agar diffusion test. Berlin, Germany:Deutsches Institut fur Normung;DIN DIN 58940-2.

Methods for the determination of susceptibility of pathogenic bacteria (without mycobacteria) to chemotherapeutic agents of discs for agar diffusion test example for getting the standard curve. Berlin, Germany:Deutsches Institut fur Normung;DIN DIN 58940-2 Beiblatt 1.

Medical microbiology - Susceptibility testing of pathogens to antimicrobial agents - Part 2: Active substance carriers for the agar diffusion test; Carrier loads and values required for drawing a standard curve. Berlin, Germany: Deutsches Institut fur Normung; DIN 58940-2 Beiblatt 2: 2002

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
  1. Accessing genome sequencing data

    Date Updated: 9/30/2019

  2. Criteria for reference quality genomes

    Date Updated: 9/30/2019