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Chlamydophila pneumoniae

VR-1360

Chlamydophila pneumoniae strain CM-1 was isolated from the sputum of a pneumonia patient in Georgia, USA. This bacterial strain is propagated in HEp-2 cells (ATCC CCL-23) and has applications in respiratory disease research.
Product category
Bacteria
Classification
Chlamydiaceae, Chlamydophila
Strain designation
CM-1
Type strain
No
Isolation source
Sputum of a pneumonia patient
Geographical isolation
United States; Georgia
Applications
Infectious disease research
Respiratory disease research
Product format
Frozen
Storage conditions
-70°C or colder
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Documentation

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Required Products

These products are vital for the proper use of this item and have been confirmed as effective in supporting functionality. If you use alternative products, the quality and effectiveness of the item may be affected.

Detailed product information

General

Specific applications
Respiratory research

Characteristics

Comments
This isolate was identified as C. pneumoniae by culture characteristics, morphology of inclusion bodies, and reaction with monoclonal antibodies by depositor. Item isolated on cycloheximide-treated HeLa 229 cells, and found to be serologically identical to the reference strain TW183 by micro-immunofluorescent test.
Mycoplasma contamination
Not detected

Handling information

Host
Effects
CPE; cytoplasmic inclusions
Complete medium
DMEM (ATCC 30-2002) + 10% prescreened FBS + 10 mM HEPES + 2 µg/mL Cycloheximide (Sigma C-4859 Ready-Made)
Temperature
36°C
Incubation
3 days
Recommendation for infection
Plate cells 24 hours prior to infection and infect when cultures are 80-95% confluent. Vortex material with 1mm glass beads to disrupt cells. Remove cell growth medium and inoculate with disrupted material. Centrifuge at 1500 x g at 25°C for 1 hour. End adsorption by adding agent growth medium.
Key abbreviations
CO2, Carbon dioxide
°C, Degrees Celsius
DMEM, Dulbecco's Modified Eagle's Medium
FBS, Fetal bovine serum
HEPES, N-(2-Hydroxyethyl)piperazine-N'-(2-ethanesulfonic acid)

Quality control specifications

Volume
1.0 mL

History

Deposited as
Chlamydia pneumoniae Grayston et al.
Depositors
KH Wong, Centers for Disease Control and Prevention
Type of isolate
Human
Special collection
NCRR Contract
Cross references
GenBank U44033 Chlamydia pneumoniae putative ribonuclease P RNA gene.

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

References

Curated Citations

Black CM, et al. Antigenic variation among strains of Chlamydia pneumoniae. J. Clin. Microbiol. 29: 1312-1316, 1991. PubMed: 1885727

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