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ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.
ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
ICD-10-CM code: C71, Malignant neoplasm of cerebellum; recurrent glioblastomaSee associated clinical data for patient profile information, if available.
If use of this culture results in a scientific publication, it should be cited in that manuscript in the following manner: HCM-BROD-0199-C71-B (ATCC® PDM-378™).
Note: This model shares a donor with PDM-143
Additionally, please acknowledge the HCMI as follows: “We used models and data derived by the Human Cancer Models Initiative (HCMI) https://ocg.cancer.gov/programs/HCMI; dbGaP accession number phs001486.”
NeuroCult NS-A Basal Medium (StemCell Technologies #05750) with NS-A Proliferation Supplement (StemCell Technologies #05754) + 20 ng/mL EGF (StemCell Technologies #78003.1) + 20 ng/mL bFGF (Peprotech #AF-100-15) + 2 µg/mL Heparin (StemCell Technologies #07980).
Prepare media according to the manufacturer’s instructions: Stem Cell Technologies Catalog #5751
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