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Penicillin-Streptomycin-Amphotericin B Solution


Penicillin-Streptomycin-Amphotericin B Solution is used to reduce the chance of microbial contamination while propagating primary cells in the culture. In general, we do not recommend the use of antibiotics and antimycotics when culturing cells as these compounds may mask contamination by susceptible bacteria and fungi while permitting mycoplasma to flourish unnoticed. In addition, these drugs may interfere with the metabolism of sensitive cells (ie, primary cells) in culture, especially when the cells are grown in low serum or serum-free environments. However, one may elect to introduce antibiotics and antimycotics to primary cultures for short periods as a safeguard.
Product category
Penicillin 10,000 Units/mL, Streptomycin 10 mg/mL, Amphotericin B 25 µg/mL
Cell growth and viability
1.0 mL
Product format
Storage conditions
-20°C or colder
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ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

Detailed product information


Specific applications
Penicillin-Streptomycin-Amphotericin B Solution is used to reduce the chance of microbial contamination while propagating primary cells or continuous cell lines in culture.

Handling information

Handling procedure
  1. Take the vial from storage and thaw. Mix well or vortex briefly to make sure the solution is homogenous.
  2. Using aseptic technique and working in a laminar flow hood or biosafety cabinet, transfer the appropriate volume of the antibiotic/antimycotic solution to complete media. 
  3. Dilute 1:1000 when using to supplement complete growth media; i.e., 0.5 mL of Penicillin-Streptomycin-Amphotericin B Solution per 500 mL of complete cell-specific media.
  4. Dilute 1:100 when using during cell isolation procedures; i.e., 0.5 mL of Penicillin-Streptomycin-Amphotericin B Solution per 50 mL of complete cell-specific growth media.
  5. Tightly cap the bottle (or other flask) of supplemented growth medium and swirl the contents gently to assure a homogeneous solution. Do not shake forcefully to avoid foaming. Label and date the flask.
  6. Fully supplemented media should be stored in the dark at 2°C to 8°C (do not freeze). When stored under these conditions, fully supplemented media is stable for 30 days.

Quality control specifications

Bacterial and fungal testing
Not detected
Mycoplasma contamination
Not detected
Functional tests
Each lot is assessed for acceptable growth of human primary cells compared to control. A Certificate of Analysis (COA) is available upon request for each lot of Penicillin-Streptomycin-Amphotericin B Solution.

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.


This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at

Frequently Asked Questions


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