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Skeletal Muscle Differentiation Tool


The Skeletal Muscle Differentiation Tool is a single-component media for the differentiation of Primary Skeletal Muscle Cells. Muscle Cells can be expanded in vitro with complete media before final differentiation into Skeletal Muscle Cells.
Product category
The Skeletal Muscle Differentiation Tool is a single component media for the differentiation of Primary Skeletal Muscle Cells (ATCC PCS-950-010). Muscle Cells can be expanded in vitro with complete media (Mesenchymal Stem Cell Media, ATCC PCS-500-030, and Skeletal Muscle Growth Kit, ATCC PCS-950-040) before final differentiation into Skeletal Muscle Cells.
Cell growth and viability
100 mL
Product format
Storage conditions
-20°C or colder
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ATCC determined that a biosafety level is not applicable to this material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to complete your own risk assessment and understand any potential hazards associated with the material per your organization’s policies and procedures and any other applicable regulations as enforced by your local or national agencies.

Required Products

These products are vital for the proper use of this item and have been confirmed as effective in supporting functionality. If you use alternative products, the quality and effectiveness of the item may be affected.

Detailed product information


Specific applications
Differentiation of Human Primary Skeletal Muscle Cells (ATCC PCS-950-010) into fused multinucleated myotubes.

Handling information

Handling procedure
  1. Seed ATCC PCS-950-010 (in complete growth media) onto a fibronectin coated multi-well plate, 50,000 cells/cm2
  2. Incubate @ 37°C for 24hrs. 
  3. Replace complete growth media with pre-warmed differentiation media after 24hrs.  
  4. Leave in differentiation media for a minimum of 96 hours or until a sufficient level of differentiation has occurred. The time to achieve differentiation will require optimization and depends on various factors including cell type, age/confluence of the culture, specific lot of smooth muscle cells, and the formulation of the differentiation medium that is used. Differentiation media should be replaced every 48 hours.

    Note: Under some conditions, optimal differentiation may require a period of 120 hours or more.

Quality control specifications

Bacterial and fungal testing
Not detected
5 EU/mL
255 to 285 mOsm/kg
7.5 to 8.1

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.


This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at

Frequently Asked Questions


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Hours of Operation

9:00am - 5:00pm
US Eastern Time