Mammary Epithelial Cell Basal Medium

Mammary Epithelial Cell Basal Medium is a sterile, phenol red-free, liquid tissue culture medium designed to support epithelial cells derived from normal human female breast. Mammary Epithelial Cell Basal Medium contains essential and non-essential amino acids, vitamins, other organic compounds, trace minerals and inorganic salts. To support the proliferation and plating efficiency of mammary epithelial cells ATCC PCS-600-010, Mammary Cell Basal Medium must be supplemented with the Mammary Epithelial Cell Growth Kit (ATCC PCS-600-040).

ATCC’s Human Mammary Epithelial Cells cultured in Mammary Epithelial Cell complete medium (basal medium plus growth kit) is an optimal serum-free culture model for many research areas. Common uses of HMEC include the study of breast cancer development, three dimensional culture and carcinogen screening.

Optional media supplements:

1. Gentamicin-Amphotericin B Solution (ATCC PCS-999-025)
2. Penicillin-Streptomycin-Amphotericin B Solution (ATCC PCS-999-002)
3. Phenol Red (ATCC PCS-999-001)

1. Obtain one growth kit from the freezer; make sure that the caps of all components are tight.
2. Thaw the components of the Mammary Epithelial Cell Growth Kit (PCS-600-040) just prior to adding them to the basal medium.
3. Obtain one bottle of Mammary Epithelial Cell Basal Medium (PCS-600-030, 485 mL) from cold storage.
4. Decontaminate the external surfaces of all growth kit component vials and the basal medium bottle by spraying them with 70% ethanol.
5. Using aseptic technique and working in a laminar flow hood or biosafety cabinet, transfer the indicated volume of each growth kit component to the bottle of basal medium using a separate sterile pipette for each transfer.

Component	Volume	Final Concentration
rH-Insulin	0.5 mL	5 µg/mL
L-Glutamine	15 mL	6 mM
Epinephrine	0.5 mL	1 µM
Apo-Transferrin	0.5 mL	5 µg/mL
rH-TGF-α	0.5 mL	0.5 ng/mL
ExtractP	2 mL	4%
Hydrocortisone Hemmisuccinate	0.5 mL	100 ng/mL

 

6. Tightly cap the bottle of complete growth medium and swirl the contents gently to assure a homogeneous solution. Do not shake forcefully to avoid foaming. Label and date the bottle.
7. Complete media should be stored in the dark at 2°C to 8°C (do not freeze). When stored under these conditions, complete media is stable for 30 days.

The product is provided 'AS IS' and the viability of ATCC^® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

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