Bladder Epithelial Cell Basal Medium

PCS-420-032 ^™

Bladder Epithelial Cell Basal Medium is a sterile, phenol red-free, liquid tissue culture medium intended for use as one component in a complete ATCC^® Primary Cell Solutions™ system. This serum-free system is designed to support bladder epithelial cells derived from normal human bladder tissue. Bladder Epithelial Cell Basal Medium contains essential and non-essential amino acids, vitamins, other organic compounds, trace minerals and inorganic salts. To support the proliferation and plating efficiency of bladder epithelial cells, medium must be supplemented with the appropriate cell-specific growth kit. When using this complete media system, the growth of bladder epithelial cells is supported without the use of feeder layers, extracellular matrix proteins or other substrates.

For bladder epithelial cells derived from bladder tissue (e.g., Primary Bladder Epithelial Cells (A/T/N), Normal, Human (ATCC PCS-420-032), supplement Bladder Epithelial Cell Basal Medium with the Bladder Epithelial Cell Growth Kit (ATCC PCS-420-042).
Optional media supplements:
- Gentamicin-Amphotericin B Solution (ATCC PCS-999-025)
- Penicillin-Streptomycin-Amphotericin B Solution (ATCC PCS-999-002)
- Phenol Red (ATCC PCS-999-001)

Product category

Reagents

Comments

Unpacking and Storage Instructions

Check all containers for leakage or breakage.
Store the growth kit(s) at either 20°C in a freezer that is not self-defrosting or at 70°C for long term storage. If thawed upon arrival, the Growth Kit can be stored at 2°C to 8°C and added to the basal medium within 72 hours of receipt.

Preparation of Complete Growth Media

Obtain one growth kit from the freezer; make sure that the caps of all components are tight.
. Thaw the components of the growth kit just prior to adding to the basal medium. Warm the L-glutamine component in a 37°C water bath and shake to dissolve any precipitates prior to adding to the basal medium.
Obtain one bottle of Vaginal Epithelial Cell Basal Medium (485 mL) from cold storage.
Decontaminate the external surfaces of all growth kit component vials and the basal medium bottle by spraying them with 70% ethanol.
Using aseptic technique and working in a laminar flow hood or biosafety cabinet, transfer the indicated volume of each growth kit component to the bottle of basal medium using a separate sterile pipette for each.

Table 1. Bladder Epithelial Cell Growth Kit Components

Component

Volume

Final Concentration

L-Glutamine

15 mL

6 mM

Extract P

2 mL

0.4%

Epinephrine

0.5 mL

1 µM

rh EGF

0.5 mL

5 ng/mL

Hydrocortisone

0.5 mL

100 ng/mL

rh Insulin

0.5 mL

5 µg/mL

Apo-transferrin

0.5 mL

5 µg/mL

rh TGF-alpha

0.5 mL

0.5 ng/mL

rh KGF

1 mL

5 ng/mL

Antimicrobials and phenol red are not required for proliferation, but may be added if desired. The recommended volume of each optional component to be added to the complete growth media is

summarized in Table 2.

Table 2. Addition of Antimicrobials/Antimycotics and Phenol Red (Optional)

Component

Volume

Final Concentration

Penicillin-Streptomycin-Amphotericin B Solution (PCS-999-002)

0.5 mL

Penicillin: 10 Units/mL

Streptomycin: 10 µg/mL

Amphotericin B: 25 ng/mL

Phenol Red (PCS-999-001)

0.5 mL

33 µM

Tightly cap the bottle of complete growth medium and swirl the contents gently to assure a homogeneous solution. Do not shake forcefully to avoid foaming. Label and date the bottle.
Complete media should be stored in the dark at 2°C to 8°C (do not freeze). When stored under these conditions, complete media is stable for 30 days.

Applications

Cell culture

Volume

485 mL

Storage conditions

2°C to 8°C

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Documentation

Product sheet

ATCC determined that a biosafety level is not applicable to this material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to complete your own risk assessment and understand any potential hazards associated with the material per your organization’s policies and procedures and any other applicable regulations as enforced by your local or national agencies.

Quality control specifications

Bacterial and fungal testing: Not detected
Endotoxin: <0.5 EU/mL
Osmolality: 255 to 285 mOsm/kg
pH: 7.5 to 8.1

Legal disclaimers

Intended use

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.

Warranty

The product is provided 'AS IS' and the viability of ATCC^® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid. Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

References

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