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Substrates and Supplements

Green and blue organoid cells.

Resources to keep your cells happy and healthy

We understand that there are no one-size-fits-all solutions in cell culture – while most cell lines have unique growth requirements we can provide the basic media reagents that many culturists need. ATCC media supplements and antibiotics allow you to customize the conditions for your primary and continuous cell lines. See our full product list below.

In addition to supplements and antibiotics, the substrate you use will affect cell growth and morphology. ATCC provides Cell Basement Membrane Gel substrate that can be used for multiple applications, such as:

  • 3D culture of primary tissue derived human and mouse organoids
  • 3D culture of iPSC derived organoids
  • 3D culture of tumorspheres from various cell lines
  • Sandwich culture of human hepatocytes
  • Feeder-free culture of human embryonic stem cells (hESC) and induced pluripotent stem cells (hiPSC)
  • Culture of iPSCs-derived neural progenitor cells (NPCs) and neurons
  • Angiogenesis modeling via the tube formation assay

Tools for successful cell culture

Purple and green Caco-2 cell junctions.

Cell Culture Guides

With nearly 100 years of expertise with cell cultures, ATCC has acquired and developed a vast body of best practices to maximize the return on your biomaterials investment.

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Clear capped and labeled bottles containing red and yellow media, ATCC products labeled: DMEM; RPMI; alpha-MEM; MCDB 131.

Media and Reagents

Our high-performance products are the same reagents we use to culture ATCC cell lines.

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Discover Best Practices for Cell Culture

Substrates & Supplements

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