Renal Epithelial Cell Basal Medium
PCS-400-030 ™
PCS-400-030 ™
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Renal Epithelial Cell Basal Medium is a sterile, phenol red-free, liquid tissue culture medium intended for use as one component in a complete ATCC® Primary Cell Solutions™ system. This system is designed to support epithelial cells (e.g., renal proximal tubule epithelial cells) derived from normal human kidney. Renal Epithelial Cell Basal Medium contains essential and non-essential amino acids, vitamins, other organic compounds, trace minerals and inorganic salts. To support the proliferation and plating efficiency of renal epithelial cells (e.g., Primary Renal Proximal Tubule Epithelial Cells, Normal, Human, ATCC PCS-400-010); Primary Renal Cortical Epithelial Cells, Normal, Human, (ATCC PCS-400-011); Primary Renal Mixed Epithelial Cells, Normal, Human, ATCC PCS-400-012), Renal Cell Basal Medium must be supplemented with the Renal Epithelial Cell Growth Kit (ATCC PCS-400-040).
Using Renal Epithelial Cell Basal Medium supplemented with the Renal Epithelial Cell Growth Kit, the growth of renal epithelial cells is supported without the use of feeder layers, extracellular matrix proteins or other substrates.
Optional media supplements:
Table 1. Renal Epithelial Cell Growth Kit Components
Component
|
Volume
|
Final Concentration
|
Fetal Bovine Serum (FBS)
|
2.5 mL
|
0.5%
|
Triiodothyronine
|
0.5 mL
|
10 nM
|
rh EGF
|
1.0 mL
|
10 ng/mL
|
Hydrocortisone Hemisuccinate
|
0.5 mL
|
100 ng/mL
|
rh Insulin
|
0.5 mL
|
5 mg/ml
|
Epinephrine
|
0.5 mL
|
1.0 mM
|
Transferrin
|
0.5 mL
|
5 mg/ml
|
L-Alanyl-L-Glutamine
|
6.0 mL
|
2.4 mM
|
Antimicrobials and phenol red are not required for proliferation but may be added if desired. The recommended volume of each optional component to be added to the complete media is summarized in Table 2.
Table 2. Addition of Antimicrobials/Antibiotics and Phenol Red (Optional)
Component
|
Volume
|
Final Concentration
|
Gentamicin-Amphotericin B Solution
|
0.5 mL
|
Gentamicin: 10 µg/mL
Amphotericin B: 0.25 µg/mL
|
Penicillin-Streptomycin-Amphotericin B Solution
|
0.5 mL
|
Penicillin: 10 Units/mL
Streptomycin: 10 µg/mL
Amphotericin B: 25 ng/mL
|
Phenol Red
|
0.5 mL
|
33 µM
|
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