Microvascular Endothelial Cell Growth Kit-BBE
PCS-110-040™
Microvascular Endothelial Cell Growth Kit-BBE (ATCC PCS-110-040) and Microvascular Endothelial Cell Growth Kit-VEGF (ATCC PCS-110-041) each contain components that when added to Vascular Cell Basal Medium (ATCC PCS-100-030) create a complete ATCC® Primary Cell Solution™ culture environment for microvascular endothelial cells derived from normal human tissue (e.g., Primary Dermal Microvascular Endothelial Cells; Normal, Human, Neonatal, ATCC PCS-110-010).
Your experimental design will dictate which Microvascular Endothelial Cell Growth Kit should be used. Use of the Microvascular Endothelial Cell Growth Kit-VEGF (ATCC PCS-110-041) will support a faster rate of proliferation because of the presence of several purified human recombinant (rh) growth factors (rh VEGF, rh EGF, rh FGF basic and rh IGF-1) combined with heparin and hydrocortisone. Use of the Microvascular Endothelial Cell Growth Kit-BBE (ATCC PCS-110-040), which contains Bovine Brain Extract (BBE), is recommended if a less defined cell culture medium is desired.